【结 构 式】 |
【分子编号】10270 【品名】7-[[Amino(imino)methyl]amino]heptanamide 【CA登记号】 |
【 分 子 式 】C8H18N4O 【 分 子 量 】186.25728 【元素组成】C 51.59% H 9.74% N 30.08% O 8.59% |
合成路线1
该中间体在本合成路线中的序号:(XI)The synthesis of racemic 15-deoxyspergualin and tritium-labeled compound has been described: The oxidation of N-(3-hydroxypropyl)carbamic acid tert-butyl ester (I) with pyridinium chlorochromate in dichloromethane gives N-(3-oxopropyl)carbamic acid tert-butyl ester (II), which is condensed with 4-(triphenylphosphonium)butyrate (III) by means of lithium bis(trimethylsilyl)amide in THF yielding 7-(tert-butoxycarbonylamino)-4-heptenoic acid (IV). The esterification of (IV) with N-hydroxysuccinimide (V) by means of dicyclohexylcarbodiimide and 1-hydroxybenzotriazole affords the corresponding ester (VI), which is treated with ammonia in methanol giving the corresponding amide (VII). The hydrolysis of (VII) with HCl in ethyl acetate yields 7-amino-4-heptenamide (VIII), which is treated with 3,5-dimethylpyrazole-1-carboxamidine (IX) and K2CO3 in methanol affording 7-guanidino-4-heptenamide (X). The reduction of (X) gives 7-guanidinoheptanamide (XI), which is finally condensed with glyoxylspermidine (XII) by means of glutaric acid in hot water. The tritium-labeled compound can also be obtained in the same way by performing the reduction of heptenamide (X) with tritium gas.
【1】 Cook, D.J.; Tepper, M.A.; Saulnier, M.G.; Dischino, D.D.; Synthesis of tritium labeled (±) 15-deoxyspergualin trihydrochloride. J Label Compd Radiopharm 1993, 33, 2, 137. |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(I) | 10260 | tert-butyl N-(3-hydroxypropyl)carbamate | 58885-58-8 | C8H17NO3 | 详情 | 详情 |
(II) | 10261 | tert-butyl N-(3-oxopropyl)carbamate | C8H15NO3 | 详情 | 详情 | |
(III) | 10262 | 4-(triphenylphosphonio)butanoate | C22H22O2P | 详情 | 详情 | |
(IV) | 10263 | (E)-7-[(tert-Butoxycarbonyl)amino]-4-heptenoic acid | C12H21NO4 | 详情 | 详情 | |
(V) | 10264 | 1-Hydroxydihydro-1H-pyrrole-2,5-dione; N-Hydroxysuccinimide; 1-Hydroxy-2,5-pyrrolidinedione | 6066-82-6 | C4H5NO3 | 详情 | 详情 |
(VI) | 10265 | tert-butyl N-[(E)-7-[(2,5-dioxo-1-pyrrolidinyl)oxy]-7-oxo-3-heptenyl]carbamate | C16H24N2O6 | 详情 | 详情 | |
(VII) | 10266 | tert-butyl N-[(E)-7-amino-7-oxo-3-heptenyl]carbamate | C12H22N2O3 | 详情 | 详情 | |
(VIII) | 10267 | (E)-7-Amino-4-heptenamide | C7H14N2O | 详情 | 详情 | |
(IX) | 10268 | 3,5-Dimethyl-1H-pyrazole-1-carboximidamide | C6H10N4 | 详情 | 详情 | |
(X) | 10269 | (E)-7-[[Amino(imino)methyl]amino]-4-heptenamide | C8H16N4O | 详情 | 详情 | |
(XI) | 10270 | 7-[[Amino(imino)methyl]amino]heptanamide | C8H18N4O | 详情 | 详情 | |
(XI) | 44635 | 7-[[amino(imino)methyl]amino]heptanamide | C8H18N4O | 详情 | 详情 | |
(XII) | 10271 | N-[4-[(3-Aminopropyl)amino]butyl]-2,2-dihydroxyacetamide | C9H21N3O3 | 详情 | 详情 |
合成路线2
该中间体在本合成路线中的序号:(V)The synthesis of [14C]-labeled 15-deoxyspergualin trihydrochloride has been reported: The condensation of [14C]-labeled S-methylisothiourea (I) with 7-aminoheptanoic acid (II) by means of NaOH in water gives 7-guanidinoheptanoic acid (III), which is treated with Dowex 50W resin (H+ form) in refluxing methanol yielding the corresponding methyl ester (IV). The reaction of (IV) with concentrated NH4OH affords the amide (V), which is finally condensed with glyoxyloxyspermidine (VI) by means of glutaric acid in hot water (60 C), and purified by chromatography over Sephadex CM-25 (Na+ form).
【1】 Dischino, D.D.; Synthesis of carbon-14 labeled (±) 15-deoxyspergualin trihydrochloride. J Label Compd Radiopharm 1995, 36, 11, 1097. |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(I) | 10272 | [[Amino(imino)methyl]sulfanyl]methane | 2986-19-8 | C2H6N2S | 详情 | 详情 |
(I) | 44636 | [[amino(imino)methyl]sulfanyl]methane | C2H6N2S | 详情 | 详情 | |
(II) | 10273 | 7-Aminoheptanoic acid | 929-17-9 | C7H15NO2 | 详情 | 详情 |
(III) | 10274 | 7-[[Amino(imino)methyl]amino]heptanoic acid | C8H17N3O2 | 详情 | 详情 | |
(III) | 44637 | 7-[[amino(imino)methyl]amino]heptanoic acid | C8H17N3O2 | 详情 | 详情 | |
(IV) | 10275 | methyl 7-[[amino(imino)methyl]amino]heptanoate | C9H19N3O2 | 详情 | 详情 | |
(IV) | 44638 | methyl 7-[[amino(imino)methyl]amino]heptanoate | C9H19N3O2 | 详情 | 详情 | |
(V) | 10270 | 7-[[Amino(imino)methyl]amino]heptanamide | C8H18N4O | 详情 | 详情 | |
(V) | 44639 | 7-[[amino(imino)methyl]amino]heptanamide | C8H18N4O | 详情 | 详情 | |
(VI) | 10271 | N-[4-[(3-Aminopropyl)amino]butyl]-2,2-dihydroxyacetamide | C9H21N3O3 | 详情 | 详情 |
合成路线3
该中间体在本合成路线中的序号:(IX)2) By an acid catalyzed condensation of 7-guanidinoheptanamide (IX) with an equivalent amount of glyoxylspermidine (X) in the presence of glutaric acid at 60 C for 8 h. Purification of the reaction mixture gives DSG in 39% yield.
【1】 Umezawa, H.; Kuroda, H.; Nakamura, T.; Umeda, Y.; Iinuma, H.; Moriguchi, M.; Takeuchi, T.; Synthesis and antitumor activity of spergualin analogues. I. Chemical modification of 7-guanidino-3-hydroxyacyl moiety. J Antibiot 1985, 38, 7, 886-898. |
【2】 Kondo, S.; Ikeda, Y.; Ikeda, D.; Iwasawa, H.; Umezawa, H.; Iinuma, H.; Umeda, Y.; The total synthesis of spergualin, an antitumor antibiotic. J Antibiot 1981, 34, 12, 1625-1627. |
【3】 Umezawa, H.; Takeuchi, T.; Kondo, S. (Microbial Chemistry Research Foundation); (-)-15-Deoxyspergualin, process for the preparation thereof, and intermediate of the same. US 4525299 . |
【4】 Fujii, A.; Iinuma, H.; Ikeda, D.; Kondo, S.; Nakamura, T.; Takeuchi, T.; Umezawa, H. (Microbial Chemistry Research Foundation); N-[4-(3-Aminopropyl)-aminobutyl]-2-(omega-guanidino-fatty-acid-amido)-2-substd.-ethanamide and salt thereof. BE 0894651; US 4518532; US 4658058 . |
【5】 Inokai, K.; Fujii, A.; Takeuchi, T.; Umeda, Y.; Moriguchi, M.; Nakamura, T.; Umezawa, H. (Nippon Kayaku Co., Ltd.; Takara Shuzo Co., Ltd.); Spegualin-related nitrile cpds. and their preparation method. JP 1985178853 . |
【6】 Suzuki, M.; Nishizawa, R.; Takei, M.; Yoshida, M. (Nippon Kayaku Co., Ltd.); Purification method of spergualin or its derivs.. JP 1984029652 . |
【7】 Nakamura, T.; Moriguchi, M.; Inokai, K.; Umeda, Y.; Umezawa, H.; Takeuchi, T.; Fujii, A. (Nippon Kayaku Co., Ltd.; Takara Shuzo Co., Ltd.); Adduct of omega-guanidino fatty acid amide and glyoxylic acid, and its preparation method. JP 1985178852 . |
【8】 Takeuchi, T.; Umezawa, H.; Kondo, S.; Nakamura, T.; Iinuma, H.; Ikeda, T.; Fujii, A. (Microbial Chemistry Research Foundation); (S)-7-Guanidino-3-hydroxyheptanamide and its synthesis method. JP 1982188562 . |
【9】 Ikeda, D.; Nakamura, T.; Fujii, A.; Umezawa, H.; Kondo, S.; Iinuma, H.; Takeuchi, T. (Microbial Chemistry Research Foundation); N-[4-(3-Aminopropyl)aminobutyl]-2,2-dihydroxyethanamide and process for its synthesis. DE 3217693; GB 2100253 . |
【10】 Takeuchi, T.; Fujii, A.; Nakamura, T.; Umeda, Y.; Moriguchi, M.; Umezawa, H. (Nippon Kayaku Co., Ltd.; Takara Shuzo Co., Ltd.); Method for producing glyoxylspermidine and the use thereof for the production of 15-deoxy spergualin-related cpds.. DE 3506330; US 4603015 . |
【11】 Cheng, C.C.; Zee-Cheng, R.K.-Y.; Deoxyspergualin. Drugs Fut 1987, 12, 2, 113. |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(V) | 10274 | 7-[[Amino(imino)methyl]amino]heptanoic acid | C8H17N3O2 | 详情 | 详情 | |
(VI) | 10273 | 7-Aminoheptanoic acid | 929-17-9 | C7H15NO2 | 详情 | 详情 |
(VII) | 22892 | N-(4-aminobutyl)-N-(3-aminopropyl)amine; N(1)-(3-aminopropyl)-1,4-butanediamine | 124-20-9 | C7H19N3 | 详情 | 详情 |
(VIII) | 22893 | 2,2-diethoxyacetic acid | C6H12O4 | 详情 | 详情 | |
(IX) | 10270 | 7-[[Amino(imino)methyl]amino]heptanamide | C8H18N4O | 详情 | 详情 | |
(X) | 10271 | N-[4-[(3-Aminopropyl)amino]butyl]-2,2-dihydroxyacetamide | C9H21N3O3 | 详情 | 详情 |