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【结 构 式】 
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【分子编号】13356 【品名】ethyl 6-amino-4-[[(1S,2R)-2-hydroxy-1-methyl-2-phenylethyl]amino]-5-nitro-2-pyridinylcarbamate 【CA登记号】 |
【 分 子 式 】C17H21N5O5 【 分 子 量 】375.38444 【元素组成】C 54.39% H 5.64% N 18.66% O 21.31% |
合成路线1
该中间体在本合成路线中的序号：(III)Synthesis of CI-980: The condensation of N-(2-amino-4-chloro-3-nitropyridin-6-yl)carbamic acid ethyl ester (I) with (1R,2S)-2-amino-1-phenyl-1-propanol (II) by means of triethylamine in refluxing ethanol gives (1S,2R)-N-[2-amino-4-(2-hydroxy-1-methyl-2-phenylethylamino)-3-nitropyridin-6-yl]carbamic acid ethyl ester (III), which is oxidized with CrO3-pyridine in dichloromethane yielding the corresponding ketone (IV). The reductocyclization of (IV) with H2 over RaNi in glacial acetic acid affords the free base of title compound (V), which is finally treated with 2-hydroxyethanesulfonic acid in methanol.
| 【1】 Graul, A.; Martell, A.M.; Castaner, J.; Mivobulin Isethionate. Drugs Fut 1997, 22, 9, 980. |
| 【2】 Carroll, T.G. Jr. (Southern Research Institute); (2S)-(5-Amino-1,2-dihydro-2-methyl-3-phenyl-pyrido[3,4-b]pyrazin-7-yl)-carbamic acid, ethyl ester. EP 0336345; JP 1989305087; US 4866059 . |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 13354 | ethyl 6-amino-4-chloro-5-nitro-2-pyridinylcarbamate | C8H9ClN4O4 | 详情 | 详情 | |
| (II) | 13355 | 2-Amino-1-phenyl-1-propanol; (1S,2R)-(+)-Norephedrine | 37577-28-9 | C9H13NO | 详情 | 详情 |
| (III) | 13356 | ethyl 6-amino-4-[[(1S,2R)-2-hydroxy-1-methyl-2-phenylethyl]amino]-5-nitro-2-pyridinylcarbamate | C17H21N5O5 | 详情 | 详情 | |
| (IV) | 13357 | ethyl 6-amino-4-[[(1S)-1-methyl-2-oxo-2-phenylethyl]amino]-5-nitro-2-pyridinylcarbamate | C17H19N5O5 | 详情 | 详情 | |
| (V) | 13358 | ethyl (2S)-5-amino-2-methyl-3-phenyl-1,2-dihydropyrido[3,4-b]pyrazin-7-ylcarbamate | C17H19N5O2 | 详情 | 详情 | |
| (VI) | 13359 | 2-Hydroxy-1-ethanesulfonic acid | 107-36-8 | C2H6O4S | 详情 | 详情 |
合成路线2
该中间体在本合成路线中的序号：(XVII)Synthesis of [14C]-labeled CI-980: The reaction of N-(ethoxycarbonyl)-L-alanine (VII) with N-methoxy-N-methylamine (VIII) by means of carbonyldiimidazole (CDI) in THF/dichloromethane gives the corresponding amide (IX), which is treated with phenylmagnesium bromide (1 mol) yielding the bromomagnesium salt (X). The bromination of [14C]-labeled benzene (XI) with Br2/HBr/H2O2 gives the labeled bromobenzene (XII), which is converted into the corresponding Grignard reagent (XIII) by reaction with Mg and dibromoethane in ether. The reaction of the previously obtained magnesium salt of alaninamide (X) with the labeled Grignard reagent (XIII) affords the labeled carbamate (XIV), which is reduced with NaBH4 in methanol and treated with KOH giving a mixture of the oxazolidinone (XV) and the aminoalcohol (XVI), separated by acidic extraction. The hydrolysis of the oxazolidinone (XV) with KOH yielded the desired aminoalcohol (XVI). The aminoalcohol (XVI) by means of triethylamine in refluxing ethanol gives (1S,2R)-N-[2-amino-4-(2-hydroxy-1-methyl-2-phenylethylamino)-3-nitropyridin-6-yl]carbamic acid ethyl ester (XVII), which is oxidized with CrO3-pyridine in dichloromethane yielding the corresponding ketone (XVIII). The reductocyclization of (XVIII) with H2 over RaNi in glacial acetic acid affords the free base of title compound (XIX), which is finally treated with 2-hydroxyethanesulfonic acid in methanol.
| 【1】 Graul, A.; Martell, A.M.; Castaner, J.; Mivobulin Isethionate. Drugs Fut 1997, 22, 9, 980. |
| 【2】 Woo, P.W.K.; Lee, H.T.; Synthesis of [14C]CI-980, ethyl [5-amino-1,2-dihydro-2(S)-methyl-3-[14C]phenylpyrido[3,4-b]pyrazin-7 -yl]carbamate isethionate salt, a tubulin-binding, antimitotic, broad-spectrum antitumor agent. J Label Compd Radiopharm 1994, 34, 1, 1-10. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (VI) | 13359 | 2-Hydroxy-1-ethanesulfonic acid | 107-36-8 | C2H6O4S | 详情 | 详情 |
| (VII) | 13360 | (2S)-2-[(Ethoxycarbonyl)amino]propionic acid | C6H11NO4 | 详情 | 详情 | |
| (VIII) | 13361 | (Methoxyamino)methane; N,O-Dimethylhydroxylamine | 1117-97-1 | C2H7NO | 详情 | 详情 |
| (IX) | 13358 | ethyl (2S)-5-amino-2-methyl-3-phenyl-1,2-dihydropyrido[3,4-b]pyrazin-7-ylcarbamate | C17H19N5O2 | 详情 | 详情 | |
| (IX) | 13362 | ethyl (1S)-2-[methoxy(methyl)amino]-1-methyl-2-oxoethylcarbamate | C8H16N2O4 | 详情 | 详情 | |
| (X) | 13363 | N-(Ethoxycarbonyl)-N-[1(S)-(N-methoxy-N-methylcarbamoyl)ethyl]amide anion | C8H15N2O4 | 详情 | 详情 | |
| (XI) | 13364 | Benzene | 71-43-2 | C6H6 | 详情 | 详情 |
| (XI) | 44622 | C6H6 | 详情 | 详情 | ||
| (XII) | 13365 | Monobromobenzene; 1-Bromobenzene；Phenylbromide;bromobenzene | 108-86-1 | C6H5Br | 详情 | 详情 |
| (XII) | 45166 | C6H5Br | 详情 | 详情 | ||
| (XIII) | 17616 | bromo(phenyl)magnesium; Phenyl Magnesium Bromide | 100-58-3 | C6H5BrMg | 详情 | 详情 |
| (XIII) | 45167 | C6H5BrMg | 详情 | 详情 | ||
| (XIV) | 13367 | ethyl (1S)-1-methyl-2-oxo-2-phenylethylcarbamate | C12H15NO3 | 详情 | 详情 | |
| (XIV) | 45168 | C12H15NO3 | 详情 | 详情 | ||
| (XV) | 13368 | (4S)-4-Methyl-5-phenyl-1,3-oxazolidin-2-one; (4S,5R)-(-)-4-Methyl-5-phenyl-2-oxazolidinone | 16251-45-9 | C10H11NO2 | 详情 | 详情 |
| (XV) | 45169 | C9H13NO | 详情 | 详情 | ||
| (XVI) | 13355 | 2-Amino-1-phenyl-1-propanol; (1S,2R)-(+)-Norephedrine | 37577-28-9 | C9H13NO | 详情 | 详情 |
| (XVI) | 45170 | C10H11NO2 | 详情 | 详情 | ||
| (XVII) | 13356 | ethyl 6-amino-4-[[(1S,2R)-2-hydroxy-1-methyl-2-phenylethyl]amino]-5-nitro-2-pyridinylcarbamate | C17H21N5O5 | 详情 | 详情 | |
| (XVII) | 63746 | C17H21N5O5 | 详情 | 详情 | ||
| (XVIII) | 13357 | ethyl 6-amino-4-[[(1S)-1-methyl-2-oxo-2-phenylethyl]amino]-5-nitro-2-pyridinylcarbamate | C17H19N5O5 | 详情 | 详情 | |
| (XVIII) | 63747 | C17H19N5O5 | 详情 | 详情 | ||
| (XIX) | 63748 | C17H19N5O2 | 详情 | 详情 |