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【结 构 式】 
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【分子编号】69221 【品名】(3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-hydroxypyrrolidine-1-carboxylate 【CA登记号】 |
【 分 子 式 】C14H26N2O5 【 分 子 量 】302.371 【元素组成】C 55.61% H 8.67% N 9.26% O 26.46% |
合成路线1
该中间体在本合成路线中的序号：(XXVIII)Protection of 3-pyrroline (XII) with Boc2O in MeOH followed by oxidation of the resulting N-Boc-pyrroline (XIII) with m-CPBA in CH2Cl2 provides epoxide (XIV), which is also alternatively prepared by reaction of 1-Boc-3-pyrroline (XIII) with NBS in aqueous DMSO and subsequent cyclization of the obtained bromohydrin (XV) in 1 N NaOH. A further method to prepare pyrroline (XIII) is by condensation of cis-1,4-dichloro-2-butene (XVI) with tert-butyl carbamate in the presence of NaH in dry DMF. Ring opening of epoxide (XIV) with NaN3 in dioxane/H2O leads to the trans-azidoalcohol (XVII), which is alkylated with iodomethane and NaH in THF to give the methyl ether (XVIII). After catalytic hydrogenation of azide (XVIII) over Pd/C, the N-Boc protecting group in the resulting amine (XIX) is replaced with a benzyl group by acidic hydrolysis followed by alkylation with benzyl chloride and Et3N to give the protected pyrrolidine (XX) . Optical resolution of racemic trans-3-amino-1-benzyl-4-methoxypyrrolidine (XX) using D-(+)-tartaric acid provides the target (S,S)-enantiomer, which is then protected as the tert-butyl carbamate (XXI) by treatment with Boc2O in MeOH. Subsequent reduction of carbamate (XXI) with LiAlH4 in THF followed by reprotection of the resulting Nmethylamine with Boc2O in CH2Cl2 provides compound (XXII), which is finally debenzylated by catalytic hydrogenation over Pd/C in EtOH to the desired pyrrolidine (VIII) .
Epoxide (XIV) is converted to the aminopyrrolidine (X) by two additional strategies. Ring opening of epoxide (XIV) with aqueous methylamine yields the racemic trans-aminoalcohol (XXIII), which is resolved by acylation with N-Boc-L-phenylalanine and EDC in CH2Cl2 followed by separation of the diastereomers by column chromatography. Hydrolysis of the desired diastereomer (XXIV) with 20% NaOH in EtOH affords the (S,S)-enantiomer of amino alcohol (XXIII), which is protected as the bis-carbamate (XXV) using Boc2O in MeOH. Finally, methylation of alcohol (XXV) with iodomethane and NaH in DMF, followed by removal of both Boc groups with p-TsOH in i-PrOH provides the target (S,S)-3-methoxy-4-(methylamino)pyrrolidine ditosylate (X) .
Alternatively, reaction of epoxide (XIV) with benzylamine provides the racemic amino alcohol (XXVI), which can be resolved by recrystallization of the diastereoisomeric salts with (+)-mandelic acid in acetonitrile/water. Subsequent debenzylation of the target enantiomer with H2 and Pd/C in BuOH provides the (S,S)-amino alcohol (XXVII). Protection of the primary amino group of compound (XXVII) with Boc2O in EtOH gives the bis-carbamate (XXVIII), which is finally submitted to N,O-dialkylation with iodomethane and NaH, followed by Boc group cleavage with p-TsOH in THF/MeOH to yield the key intermediate (X) .
| 【1】 Tomita, K., Chiba, K., Kashimoto, S., Shibamori, K., Tsuzuki, Y. (Dainippon Sumitomo Pharma Co., Ltd.). Novel compound, process for producing the same, and antitumor agent. EP 0787726, US 5817669, WO 1995034559. |
| 【2】 Tsuzuki, Y., Chiba, K., Mizuno, K., Tomita, K., Suzuki, K. Practical synthesis of (3S,4S)-3-methoxy-4-methylaminopyrrolidine. Tetrahedron Asymmetry 2001, 12(21): 2989-97. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| （LVIV） | 69218 | (S,S)-tert-butyl 3-hydroxy-4-(methylamino)pyrrolidine-1-carboxylate | C10H20N2O3 | 详情 | 详情 | |
| (VIII) | 26649 | tert-butyl (3S,4S)-4-methoxypyrrolidinyl(methyl)carbamate | C11H22N2O3 | 详情 | 详情 | |
| (X) | 69211 | (3S,4S)-4-methoxy-N-methylpyrrolidin-3-amine compound with 1-methyl-4-(methylsulfonyl)benzene (1:2) | C6H14N2O.2C8H10O2S | 详情 | 详情 | |
| (XII) | 22833 | 2,5-dihydro-1H-pyrrole;3-Pyrroline | 109-96-6 | C4H7N | 详情 | 详情 |
| (XIII) | 22834 | 2,5-Dihydro-1H-pyrrole-1-carboxylic acid 1,1-d;2,5-Dihydropyrrole-1-carboxylic acid tert-butyl ester;tert-Butyl 2H-pyrrole-1(5H)-carboxylate;1-tert-Butoxycarbonylpyrroline;tert-butyl 2,5-dihydro-1H-pyrrole-1-carboxylate | 73286-70-1 | C9H15NO2 | 详情 | 详情 |
| (XIV) | 22835 | tert-butyl 6-oxa-3-azabicyclo[3.1.0]hexane-3-carboxylate | C9H15NO3 | 详情 | 详情 | |
| (XV) | 69212 | (3S,4R)-tert-butyl 3-bromo-4-hydroxypyrrolidine-1-carboxylate | C9H16BrNO3 | 详情 | 详情 | |
| (XVI) | 50004 | cis-1,4-Dichloro-2-butene;(Z)-1,4-Dichlorobutene;(Z)-1,4-Dichloro-2-butene;(2Z)-1,4-Dichloro-2-butene;cis-1,2-Bis(chloromethyl)ethene | 1476-11-5 | C4H6Cl2 | 详情 | 详情 |
| (XVII) | 69213 | (3S,4S)-tert-butyl 3-azido-4-hydroxypyrrolidine-1-carboxylate | C9H16N4O3 | 详情 | 详情 | |
| (XVIII) | 69214 | (3S,4S)-tert-butyl 3-azido-4-methoxypyrrolidine-1-carboxylate | 429673-78-9 | C10H18N4O3 | 详情 | 详情 |
| (XIX) | 69215 | (3S,4S)-tert-butyl 3-amino-4-methoxypyrrolidine-1-carboxylate | 429673-79-0 | C10H20N2O3 | 详情 | 详情 |
| (XX) | 26651 | (3S,4S)-1-benzyl-4-methoxy-3-pyrrolidinamine | C12H18N2O | 详情 | 详情 | |
| (XXI) | 26652 | tert-butyl (3S,4S)-1-benzyl-4-methoxypyrrolidinylcarbamate | C17H26N2O3 | 详情 | 详情 | |
| (XXII) | 26653 | tert-butyl (3S,4S)-1-benzyl-4-methoxypyrrolidinyl(methyl)carbamate | C18H28N2O3 | 详情 | 详情 | |
| (XXIII) | 69216 | rac-tert-butyl 3-hydroxy-4-(methylamino)pyrrolidine-1-carboxylate | C10H20N2O3 | 详情 | 详情 | |
| (XXIV) | 69217 | (3S,4S)-tert-butyl 3-((S)-2-((tert-butoxycarbonyl)amino)-N-methyl-3-phenylpropanamido)-4-hydroxypyrrolidine-1-carboxylate | C24H37N3O6 | 详情 | 详情 | |
| (XXV) | 69219 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)(methyl)amino)-4-hydroxypyrrolidine-1-carboxylate | C15H28N2O5 | 详情 | 详情 | |
| (XXVI) | 69220 | (3S,4S)-tert-butyl 3-(benzylamino)-4-hydroxypyrrolidine-1-carboxylate | 252574-03-1 | C16H24N2O3 | 详情 | 详情 |
| (XXVII) | 22837 | tert-butyl (3S,4S)-3-amino-4-hydroxy-1-pyrrolidinecarboxylate | 190792-74-6 | C9H18N2O3 | 详情 | 详情 |
| (XXVIII) | 69221 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-hydroxypyrrolidine-1-carboxylate | C14H26N2O5 | 详情 | 详情 |
合成路线2
该中间体在本合成路线中的序号：(XXVIII)Stereospecific methods to prepare aminopyrrolidine (X) have been developed starting from both enantiomers of trans-N-benzyl-2,3-dihydroxysuccinimide (XXIXa) and (XXIXb), generated respectively from D- and L-tartaric acid. Monoprotection of the (S,S)-diol (XXIXa) with one equivalent of TBDMSCl in the presence of imidazole in cold DMF followed by imide reduction with borane-THF complex leads to the pyrrolidine (XXXa), which after debenzylation with H2 and Pd/C and reprotection with Boc2O provides the tert-butyl carbamate (XXXIa). Alcohol (XXXIa) is then converted to the corresponding mesylate, which undergoes nucleophilic displacement by NaN3 in DMF to give the cis-azide (XXXII). Azide (XXXII) is then reduced by catalytic hydrogenation over Pd/C to afford the corresponding amine, which is protected with Boc2O, followed by desilylation with tetrabutylammonium fluoride to give the cis-alcohol (XXXIII). Inversion of the configuration of alcohol (XXXIII) to the trans-isomer (XXVIII) is then accomplished by mesylation with MsCl and Et3N followed by displacement with potassium acetate in DMF and acetate hydrolysis with K2CO3 in MeOH. Finally, compound (XXVIII) is methylated with iodomethane and NaH and subsequently deprotected with p-TsOH in i-PrOH .
Alternatively, the (R,R)-imide (XXIXb) is converted to the trans-diol derivative (XXXb) as before, and the N-benzyl group is then exchanged for a Boc group, giving alcohol (XXXIb). Alkylation of compound (XXXIb) with iodomethane and NaH affords the corresponding methyl ether, which is desilylated to alcohol (XXXIV) by means of TBAF in THF. Mesylation of alcohol (XXXIV), followed by displacement with lithium acetate produces the methoxy acetate (XXXV), which is hydrolyzed to the cis-alcohol (XXXVI) using K2CO3 in MeOH. Mesylation of alcohol (XXXVI) followed by displacement with NaN3 provides the azide (XXXVII). After reduction with H2 and Pd/C, the resulting amine is protected as the Boc derivative (XXXVIII) with Boc2O in EtOH. Finally, amine (XXXVIII) is alkylated with iodomethane and NaH, followed by deprotection with p-TsOH (X) .
| 【1】 Tsuzuku, Y., Chiba, K., Hino, K. Efficient stereospecific synthesis of (S,S)-3-methoxy-4-methylaminopyrrolidine. Tetrahedron Asymmetry 2001, 12(12): 1793-9. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (XXIXa) | 69222 | trans-N-benzyl-2,3-dihydroxysuccinimide; (3S,4S)-3,4-dihydroxy-1-(phenylmethyl)-2,5-Pyrrolidinedione; (3S-trans)-3,4-dihydroxy-1-(phenylmethyl)-2,5-Pyrrolidinedione | 187032-53-7 | C11H11NO4 | 详情 | 详情 |
| (XXIXb) | 33017 | (3R,4R)-1-benzyl-3,4-dihydroxy-2,5-pyrrolidinedione | 75172-31-5 | C11H11NO4 | 详情 | 详情 |
| (XXXa) | 69223 | (3S,4S)-1-benzyl-4-((tert-butyldimethylsilyl)oxy)pyrrolidin-3-ol | C17H29NO2Si | 详情 | 详情 | |
| (XXXb) | 69224 | (3R,4R)-1-benzyl-4-((tert-butyldimethylsilyl)oxy)pyrrolidin-3-ol | C17H29NO2Si | 详情 | 详情 | |
| (XXXIa) | 69226 | (3S,4S)-tert-butyl 3-((tert-butyldimethylsilyl)oxy)-4-hydroxypyrrolidine-1-carboxylate | C15H31NO4Si | 详情 | 详情 | |
| (XXXIb) | 69225 | (3R,4R)-tert-butyl 3-((tert-butyldimethylsilyl)oxy)-4-hydroxypyrrolidine-1-carboxylate | C15H31NO4Si | 详情 | 详情 | |
| (X) | 69211 | (3S,4S)-4-methoxy-N-methylpyrrolidin-3-amine compound with 1-methyl-4-(methylsulfonyl)benzene (1:2) | C6H14N2O.2C8H10O2S | 详情 | 详情 | |
| (XXVIII) | 69221 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-hydroxypyrrolidine-1-carboxylate | C14H26N2O5 | 详情 | 详情 | |
| (XXXII) | 69228 | (3R,4S)-tert-butyl 3-azido-4-((tert-butyldimethylsilyl)oxy)pyrrolidine-1-carboxylate | C15H30N4O3Si | 详情 | 详情 | |
| (XXXIII) | 69229 | (3R,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-hydroxypyrrolidine-1-carboxylate | C14H26N2O5 | 详情 | 详情 | |
| (XXXIV) | 69227 | (3R,4R)-tert-butyl 3-hydroxy-4-methoxypyrrolidine-1-carboxylate | C10H19NO4 | 详情 | 详情 | |
| (XXXV) | 69230 | (3S,4R)-tert-butyl 3-acetoxy-4-methoxypyrrolidine-1-carboxylate | C12H21NO5 | 详情 | 详情 | |
| (XXXVI) | 69231 | (3S,4R)-tert-butyl 3-hydroxy-4-methoxypyrrolidine-1-carboxylate | C10H19NO4 | 详情 | 详情 | |
| (XXXVII) | 69232 | (3S,4S)-tert-butyl 3-azido-4-methoxypyrrolidine-1-carboxylate;trans-3-Azido-4-methoxy-pyrrolidine-1-carboxylic acid tert-butyl ester;TRANS-3-AZIDO-1-BOC-4-METHOXYPYRROLIDINE | 429673-78-9 | C10H18N4O3 | 详情 | 详情 |
| (XXXVIII) | 69233 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-methoxypyrrolidine-1-carboxylate | C15H28N2O5 | 详情 | 详情 |