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【结 构 式】 
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【药物名称】Vosaroxin；SNS-595；SPC-595；AG-7352 【化学名称】(+)-7-[3(S)-Methoxy-4(S)-(methylamino)pyrrolidin-1-yl]-4-oxo-1-(2-thiazolyl)-1,4-dihydro-1,8-naphthyridine-3-carboxylic acid 【CA登记号】175414-77-4；175519-16-1 (hydrochloride) 【 分 子 式 】C18H19N5O4S 【 分 子 量 】401.44723 |
【开发单位】Dainippon Pharmaceutical (Originator), Sunesis (Licensee) 【药理作用】Oncolytic；DNA-Intercalating Drug；Topoisomerase II Inhibitors |
合成路线1
Treatment of 2,6-dichloropyridine (I) with butyllithium in THF followed by quenching with CO2 affords 2,6-dichloronicotinic acid (II) . After chlorination with boiling SOCl2, the resulting acid chloride (IIIa) is condensed with diethyl malonate by means of magnesium ethoxide in Et2O to give the nicotinoyl acetate (IV) . Alternatively, activation of 2,6-dichloronicotinic acid (II) with CDI provides imidazolide (IIIb) which, without isolation, is condensed with the potassium salt of diethyl malonate in the presence of MgCl2 and Et3N to yield the keto ester (IV) . Treatment of compound (IV) with triethylorthoformate and acetic anhydride followed by reaction with 2-aminothiazole (V) gives the enamino ester (VI), which is cyclized to the 1,8-naphthyridine derivative (VII) upon heating with K2CO3 in dioxane . Condensation of chloronaphthyridine (VII) with the chiral pyrrolidine (VIII) by means of triethylamine in acetonitrile yields the protected voreloxin (IX), which is finally hydrolyzed with hot aqueous HCl . In a related method, chloronaphthyridine (VII) is coupled with the unprotected pyrrolidine (X) to afford voreloxin ethyl ester (XI), which is finally hydrolyzed with NaOH in H2O/EtOH .
| 【1】 Tomita, K. et al. Synthesis and antitumor activity of novel 7-substituted 1,4-dihydro-4-oxo-1-(2-thiazolyl)-1,8-naphthyridine-3-carboxylic acids. 217th ACS Natl Meet (March 21-25, Anaheim) 1999, Abst MEDI 249. |
| 【2】 Tomita, K., Chiba, K., Kashimoto, S., Shibamori, K., Tsuzuki, Y. (Dainippon Sumitomo Pharma Co., Ltd.). Novel compound, process for producing the same, and antitumor agent. EP 0787726, US 5817669, WO 1995034559. |
| 【3】 Tsuzuki, Y., Tomita, K., Shibamori, K.-I., Sato, Y., Kashimoto, S., Chiba, K. Synthesis and structure-activity relationships of novel 7-substituted 1,4-dihydro-4-oxo-1-(2-thiazolyl)-1,8-naphthyridine-3-carboxylic acids as antitumor agents. Part 2. J Med Chem 2004, 47(8): 2097-109. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (IIIa) | 26645 | 2,6-dichloronicotinoyl chloride | C6H2Cl3NO | 详情 | 详情 | |
| (I) | 13573 | 2,6-Dichloropyridine | 2402-78-0 | C5H3Cl2N | 详情 | 详情 |
| (II) | 26644 | 2,6-dichloronicotinic acid | 38496-18-3 | C6H3Cl2NO2 | 详情 | 详情 |
| (IV) | 26646 | ethyl 3-(2,6-dichloro-3-pyridinyl)-3-oxopropanoate | C10H9Cl2NO3 | 详情 | 详情 | |
| (V) | 19795 | 2-Thiazolamine; 1,3-thiazol-2-amine; 1,3-thiazol-2-ylamine | 96-50-4 | C3H4N2S | 详情 | 详情 |
| (VI) | 26647 | ethyl (Z)-2-[(2,6-dichloro-3-pyridinyl)carbonyl]-3-(1,3-thiazol-2-ylamino)-2-propenoate | C14H11Cl2N3O3S | 详情 | 详情 | |
| (VII) | 26648 | ethyl 7-chloro-4-oxo-1-(1,3-thiazol-2-yl)-1,4-dihydro[1,8]naphthyridine-3-carboxylate | C14H10ClN3O3S | 详情 | 详情 | |
| (VIII) | 26649 | tert-butyl (3S,4S)-4-methoxypyrrolidinyl(methyl)carbamate | C11H22N2O3 | 详情 | 详情 | |
| (IX) | 26650 | ethyl 7-[(3S,4S)-3-[(tert-butoxycarbonyl)(methyl)amino]-4-methoxypyrrolidinyl]-4-oxo-1-(1,3-thiazol-2-yl)-1,4-dihydro[1,8]naphthyridine-3-carboxylate | C25H31N5O6S | 详情 | 详情 | |
| (X) | 69211 | (3S,4S)-4-methoxy-N-methylpyrrolidin-3-amine compound with 1-methyl-4-(methylsulfonyl)benzene (1:2) | C6H14N2O.2C8H10O2S | 详情 | 详情 | |
| (XI) | 69210 | ethyl 7-((3S,4S)-3-methoxy-4-(methylamino)pyrrolidin-1-yl)-4-oxo-1-(thiazol-2-yl)-1,4-dihydro-1,8-naphthyridine-3-carboxylate | C20H23N5O4S | 详情 | 详情 |
合成路线2
Protection of 3-pyrroline (XII) with Boc2O in MeOH followed by oxidation of the resulting N-Boc-pyrroline (XIII) with m-CPBA in CH2Cl2 provides epoxide (XIV), which is also alternatively prepared by reaction of 1-Boc-3-pyrroline (XIII) with NBS in aqueous DMSO and subsequent cyclization of the obtained bromohydrin (XV) in 1 N NaOH. A further method to prepare pyrroline (XIII) is by condensation of cis-1,4-dichloro-2-butene (XVI) with tert-butyl carbamate in the presence of NaH in dry DMF. Ring opening of epoxide (XIV) with NaN3 in dioxane/H2O leads to the trans-azidoalcohol (XVII), which is alkylated with iodomethane and NaH in THF to give the methyl ether (XVIII). After catalytic hydrogenation of azide (XVIII) over Pd/C, the N-Boc protecting group in the resulting amine (XIX) is replaced with a benzyl group by acidic hydrolysis followed by alkylation with benzyl chloride and Et3N to give the protected pyrrolidine (XX) . Optical resolution of racemic trans-3-amino-1-benzyl-4-methoxypyrrolidine (XX) using D-(+)-tartaric acid provides the target (S,S)-enantiomer, which is then protected as the tert-butyl carbamate (XXI) by treatment with Boc2O in MeOH. Subsequent reduction of carbamate (XXI) with LiAlH4 in THF followed by reprotection of the resulting Nmethylamine with Boc2O in CH2Cl2 provides compound (XXII), which is finally debenzylated by catalytic hydrogenation over Pd/C in EtOH to the desired pyrrolidine (VIII) .
Epoxide (XIV) is converted to the aminopyrrolidine (X) by two additional strategies. Ring opening of epoxide (XIV) with aqueous methylamine yields the racemic trans-aminoalcohol (XXIII), which is resolved by acylation with N-Boc-L-phenylalanine and EDC in CH2Cl2 followed by separation of the diastereomers by column chromatography. Hydrolysis of the desired diastereomer (XXIV) with 20% NaOH in EtOH affords the (S,S)-enantiomer of amino alcohol (XXIII), which is protected as the bis-carbamate (XXV) using Boc2O in MeOH. Finally, methylation of alcohol (XXV) with iodomethane and NaH in DMF, followed by removal of both Boc groups with p-TsOH in i-PrOH provides the target (S,S)-3-methoxy-4-(methylamino)pyrrolidine ditosylate (X) .
Alternatively, reaction of epoxide (XIV) with benzylamine provides the racemic amino alcohol (XXVI), which can be resolved by recrystallization of the diastereoisomeric salts with (+)-mandelic acid in acetonitrile/water. Subsequent debenzylation of the target enantiomer with H2 and Pd/C in BuOH provides the (S,S)-amino alcohol (XXVII). Protection of the primary amino group of compound (XXVII) with Boc2O in EtOH gives the bis-carbamate (XXVIII), which is finally submitted to N,O-dialkylation with iodomethane and NaH, followed by Boc group cleavage with p-TsOH in THF/MeOH to yield the key intermediate (X) .
| 【1】 Tomita, K., Chiba, K., Kashimoto, S., Shibamori, K., Tsuzuki, Y. (Dainippon Sumitomo Pharma Co., Ltd.). Novel compound, process for producing the same, and antitumor agent. EP 0787726, US 5817669, WO 1995034559. |
| 【2】 Tsuzuki, Y., Chiba, K., Mizuno, K., Tomita, K., Suzuki, K. Practical synthesis of (3S,4S)-3-methoxy-4-methylaminopyrrolidine. Tetrahedron Asymmetry 2001, 12(21): 2989-97. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| （LVIV） | 69218 | (S,S)-tert-butyl 3-hydroxy-4-(methylamino)pyrrolidine-1-carboxylate | C10H20N2O3 | 详情 | 详情 | |
| (VIII) | 26649 | tert-butyl (3S,4S)-4-methoxypyrrolidinyl(methyl)carbamate | C11H22N2O3 | 详情 | 详情 | |
| (X) | 69211 | (3S,4S)-4-methoxy-N-methylpyrrolidin-3-amine compound with 1-methyl-4-(methylsulfonyl)benzene (1:2) | C6H14N2O.2C8H10O2S | 详情 | 详情 | |
| (XII) | 22833 | 2,5-dihydro-1H-pyrrole;3-Pyrroline | 109-96-6 | C4H7N | 详情 | 详情 |
| (XIII) | 22834 | 2,5-Dihydro-1H-pyrrole-1-carboxylic acid 1,1-d;2,5-Dihydropyrrole-1-carboxylic acid tert-butyl ester;tert-Butyl 2H-pyrrole-1(5H)-carboxylate;1-tert-Butoxycarbonylpyrroline;tert-butyl 2,5-dihydro-1H-pyrrole-1-carboxylate | 73286-70-1 | C9H15NO2 | 详情 | 详情 |
| (XIV) | 22835 | tert-butyl 6-oxa-3-azabicyclo[3.1.0]hexane-3-carboxylate | C9H15NO3 | 详情 | 详情 | |
| (XV) | 69212 | (3S,4R)-tert-butyl 3-bromo-4-hydroxypyrrolidine-1-carboxylate | C9H16BrNO3 | 详情 | 详情 | |
| (XVI) | 50004 | cis-1,4-Dichloro-2-butene;(Z)-1,4-Dichlorobutene;(Z)-1,4-Dichloro-2-butene;(2Z)-1,4-Dichloro-2-butene;cis-1,2-Bis(chloromethyl)ethene | 1476-11-5 | C4H6Cl2 | 详情 | 详情 |
| (XVII) | 69213 | (3S,4S)-tert-butyl 3-azido-4-hydroxypyrrolidine-1-carboxylate | C9H16N4O3 | 详情 | 详情 | |
| (XVIII) | 69214 | (3S,4S)-tert-butyl 3-azido-4-methoxypyrrolidine-1-carboxylate | 429673-78-9 | C10H18N4O3 | 详情 | 详情 |
| (XIX) | 69215 | (3S,4S)-tert-butyl 3-amino-4-methoxypyrrolidine-1-carboxylate | 429673-79-0 | C10H20N2O3 | 详情 | 详情 |
| (XX) | 26651 | (3S,4S)-1-benzyl-4-methoxy-3-pyrrolidinamine | C12H18N2O | 详情 | 详情 | |
| (XXI) | 26652 | tert-butyl (3S,4S)-1-benzyl-4-methoxypyrrolidinylcarbamate | C17H26N2O3 | 详情 | 详情 | |
| (XXII) | 26653 | tert-butyl (3S,4S)-1-benzyl-4-methoxypyrrolidinyl(methyl)carbamate | C18H28N2O3 | 详情 | 详情 | |
| (XXIII) | 69216 | rac-tert-butyl 3-hydroxy-4-(methylamino)pyrrolidine-1-carboxylate | C10H20N2O3 | 详情 | 详情 | |
| (XXIV) | 69217 | (3S,4S)-tert-butyl 3-((S)-2-((tert-butoxycarbonyl)amino)-N-methyl-3-phenylpropanamido)-4-hydroxypyrrolidine-1-carboxylate | C24H37N3O6 | 详情 | 详情 | |
| (XXV) | 69219 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)(methyl)amino)-4-hydroxypyrrolidine-1-carboxylate | C15H28N2O5 | 详情 | 详情 | |
| (XXVI) | 69220 | (3S,4S)-tert-butyl 3-(benzylamino)-4-hydroxypyrrolidine-1-carboxylate | 252574-03-1 | C16H24N2O3 | 详情 | 详情 |
| (XXVII) | 22837 | tert-butyl (3S,4S)-3-amino-4-hydroxy-1-pyrrolidinecarboxylate | 190792-74-6 | C9H18N2O3 | 详情 | 详情 |
| (XXVIII) | 69221 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-hydroxypyrrolidine-1-carboxylate | C14H26N2O5 | 详情 | 详情 |
合成路线3
Stereospecific methods to prepare aminopyrrolidine (X) have been developed starting from both enantiomers of trans-N-benzyl-2,3-dihydroxysuccinimide (XXIXa) and (XXIXb), generated respectively from D- and L-tartaric acid. Monoprotection of the (S,S)-diol (XXIXa) with one equivalent of TBDMSCl in the presence of imidazole in cold DMF followed by imide reduction with borane-THF complex leads to the pyrrolidine (XXXa), which after debenzylation with H2 and Pd/C and reprotection with Boc2O provides the tert-butyl carbamate (XXXIa). Alcohol (XXXIa) is then converted to the corresponding mesylate, which undergoes nucleophilic displacement by NaN3 in DMF to give the cis-azide (XXXII). Azide (XXXII) is then reduced by catalytic hydrogenation over Pd/C to afford the corresponding amine, which is protected with Boc2O, followed by desilylation with tetrabutylammonium fluoride to give the cis-alcohol (XXXIII). Inversion of the configuration of alcohol (XXXIII) to the trans-isomer (XXVIII) is then accomplished by mesylation with MsCl and Et3N followed by displacement with potassium acetate in DMF and acetate hydrolysis with K2CO3 in MeOH. Finally, compound (XXVIII) is methylated with iodomethane and NaH and subsequently deprotected with p-TsOH in i-PrOH .
Alternatively, the (R,R)-imide (XXIXb) is converted to the trans-diol derivative (XXXb) as before, and the N-benzyl group is then exchanged for a Boc group, giving alcohol (XXXIb). Alkylation of compound (XXXIb) with iodomethane and NaH affords the corresponding methyl ether, which is desilylated to alcohol (XXXIV) by means of TBAF in THF. Mesylation of alcohol (XXXIV), followed by displacement with lithium acetate produces the methoxy acetate (XXXV), which is hydrolyzed to the cis-alcohol (XXXVI) using K2CO3 in MeOH. Mesylation of alcohol (XXXVI) followed by displacement with NaN3 provides the azide (XXXVII). After reduction with H2 and Pd/C, the resulting amine is protected as the Boc derivative (XXXVIII) with Boc2O in EtOH. Finally, amine (XXXVIII) is alkylated with iodomethane and NaH, followed by deprotection with p-TsOH (X) .
| 【1】 Tsuzuku, Y., Chiba, K., Hino, K. Efficient stereospecific synthesis of (S,S)-3-methoxy-4-methylaminopyrrolidine. Tetrahedron Asymmetry 2001, 12(12): 1793-9. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (XXIXa) | 69222 | trans-N-benzyl-2,3-dihydroxysuccinimide; (3S,4S)-3,4-dihydroxy-1-(phenylmethyl)-2,5-Pyrrolidinedione; (3S-trans)-3,4-dihydroxy-1-(phenylmethyl)-2,5-Pyrrolidinedione | 187032-53-7 | C11H11NO4 | 详情 | 详情 |
| (XXIXb) | 33017 | (3R,4R)-1-benzyl-3,4-dihydroxy-2,5-pyrrolidinedione | 75172-31-5 | C11H11NO4 | 详情 | 详情 |
| (XXXa) | 69223 | (3S,4S)-1-benzyl-4-((tert-butyldimethylsilyl)oxy)pyrrolidin-3-ol | C17H29NO2Si | 详情 | 详情 | |
| (XXXb) | 69224 | (3R,4R)-1-benzyl-4-((tert-butyldimethylsilyl)oxy)pyrrolidin-3-ol | C17H29NO2Si | 详情 | 详情 | |
| (XXXIa) | 69226 | (3S,4S)-tert-butyl 3-((tert-butyldimethylsilyl)oxy)-4-hydroxypyrrolidine-1-carboxylate | C15H31NO4Si | 详情 | 详情 | |
| (XXXIb) | 69225 | (3R,4R)-tert-butyl 3-((tert-butyldimethylsilyl)oxy)-4-hydroxypyrrolidine-1-carboxylate | C15H31NO4Si | 详情 | 详情 | |
| (X) | 69211 | (3S,4S)-4-methoxy-N-methylpyrrolidin-3-amine compound with 1-methyl-4-(methylsulfonyl)benzene (1:2) | C6H14N2O.2C8H10O2S | 详情 | 详情 | |
| (XXVIII) | 69221 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-hydroxypyrrolidine-1-carboxylate | C14H26N2O5 | 详情 | 详情 | |
| (XXXII) | 69228 | (3R,4S)-tert-butyl 3-azido-4-((tert-butyldimethylsilyl)oxy)pyrrolidine-1-carboxylate | C15H30N4O3Si | 详情 | 详情 | |
| (XXXIII) | 69229 | (3R,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-hydroxypyrrolidine-1-carboxylate | C14H26N2O5 | 详情 | 详情 | |
| (XXXIV) | 69227 | (3R,4R)-tert-butyl 3-hydroxy-4-methoxypyrrolidine-1-carboxylate | C10H19NO4 | 详情 | 详情 | |
| (XXXV) | 69230 | (3S,4R)-tert-butyl 3-acetoxy-4-methoxypyrrolidine-1-carboxylate | C12H21NO5 | 详情 | 详情 | |
| (XXXVI) | 69231 | (3S,4R)-tert-butyl 3-hydroxy-4-methoxypyrrolidine-1-carboxylate | C10H19NO4 | 详情 | 详情 | |
| (XXXVII) | 69232 | (3S,4S)-tert-butyl 3-azido-4-methoxypyrrolidine-1-carboxylate;trans-3-Azido-4-methoxy-pyrrolidine-1-carboxylic acid tert-butyl ester;TRANS-3-AZIDO-1-BOC-4-METHOXYPYRROLIDINE | 429673-78-9 | C10H18N4O3 | 详情 | 详情 |
| (XXXVIII) | 69233 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-methoxypyrrolidine-1-carboxylate | C15H28N2O5 | 详情 | 详情 |
合成路线4
Intermediate (XXXVIII) can be prepared by several alternative methods. Protection of diallylamine (XXXIX) with benzyl chloroformate and Et3N in CH2Cl2 yields the benzyloxycarbonyl derivative (XL), which undergoes ring-closing metathesis in the presence of Grubbs’ catalyst to give 1-Cbz-3-pyrroline (XLI). Reaction of compound (XLI) with NBS in aqueous DMSO followed by treatment of the resulting bromohydrin with 1 N NaOH leads to epoxide (XLII), which undergoes ring opening with NaN3 in aqueous dioxane to afford the racemic trans-azidoalcohol (XLIII). Resolution of rac-(XLIII) is then effected by incubation with lipase PS in the presence of vinyl acetate in i-Pr2O to give the (R,R)-acetate (XLIV) along with unreacted (S,S)-alcohol (XLIII). Methylation of (S,S)-alcohol (XLIII) with iodomethane and NaH affords ether (XLV), which is submitted to azide group reduction by means of PPh3 in THF/H2O to give amine (XLVI). Protection of amine (XLVI) with Boc2O and Et3N followed by selective removal of the Cbz group in the resulting bis-carbamate (XLVII) using poly(methylhydrosiloxane) and Pd/C in EtOH leads to the pyrrolidine (XLVIII), which is finally treated with Boc2O and Et3N .
| 【1】 Kamal, A., Shaik, A.A., Sandbhor, M., Malik, M.S., Kaga, H. Chemoenzymatic synthesis of (3S,4S)- and (3R,4R)-3-methoxy-4-methylaminopyrrolidine. Tetrahedron Lett 2004, 45(43): 8057-9. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (XXXVIII) | 69233 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-methoxypyrrolidine-1-carboxylate | C15H28N2O5 | 详情 | 详情 | |
| (XXXIX) | 68044 | diallylamine;Di-2-propenylamine;N-2-Propenyl-2-propen-1-amine | 124-02-7 | C6H11N | 详情 | 详情 |
| (XL) | 69234 | benzyl diallylcarbamate | C14H17NO2 | 详情 | 详情 | |
| (XLI) | 15923 | 1-Benzyloxycarbonyl-3-pyrroline;3-Pyrroline-1-carboxylicacid, benzyl ester ;N-(Benzyloxycarbonyl)-3-pyrroline;Benzyl 3-pyrroline-1-carboxylate;2H,5H-Pyrrole-1-carboxylicacid benzyl ester;2,5-Dihydropyrrole-1-carboxylic acid benzyl ester;benzyl 2,5-dihydro-1H-pyrrole-1-carboxylate | 31970-04-4 | C12H13NO2 | 详情 | 详情 |
| (XLII) | 69235 | benzyl 6-oxa-3-azabicyclo[3.1.0]hexane-3-carboxylate | C12H13NO3 | 详情 | 详情 | |
| (XLIII) | 69236 | (3S,4S)-benzyl 3-azido-4-hydroxypyrrolidine-1-carboxylate-benzyl 3-azido-4-hydroxypyrrolidine-1-carboxylate | C12H14N4O3 | 详情 | 详情 | |
| (XLIV) | 69237 | (3R,4R)-benzyl 3-acetoxy-4-azidopyrrolidine-1-carboxylate | C14H16N4O4 | 详情 | 详情 | |
| (XLV) | 69238 | (3S,4S)-benzyl 3-azido-4-methoxypyrrolidine-1-carboxylate | C13H16N4O3 | 详情 | 详情 | |
| (XLVI) | 69241 | (3S,4S)-benzyl 3-amino-4-methoxypyrrolidine-1-carboxylate | C13H18N2O3 | 详情 | 详情 | |
| (XLVII) | 69239 | (3S,4S)-benzyl 3-((tert-butoxycarbonyl)amino)-4-methoxypyrrolidine-1-carboxylate | C18H26N2O5 | 详情 | 详情 | |
| (XLVIII) | 69240 | tert-butyl ((3S,4S)-4-methoxypyrrolidin-3-yl)carbamate | C10H20N2O3 | 详情 | 详情 |
合成路线5
Treatment of the known diol (XLIX) with tosyl chloride in cold pyridine gives the primary tosylate (L), which is reacted with NaN3 in DMF to give azidoalcohol (LI). Subsequent alkylation of the secondary alcohol (LI) with MeI and NaH followed by acidic ketal hydrolysis of the resulting methyl ether (LII) affords the diol (LIII), which is converted to ditosylate (LIV) using p-TsCl and Et3N. Reduction of azide (LIV) using PPh3 in refluxing MeOH occurs with concomitant cyclization to give the pyrrolidine (LV), which is then protected as the N-Boc derivative (LVI) under standard conditions. The remaining tosylate group of (LVI) is then displaced with NaN3 in DMF to furnish azide (LVII). After reduction by catalytic hydrogenation over Pd/C, the obtained amine (LVIII) is finally protected with Boc2O and Et3N in CH2Cl2 .
| 【1】 Kumar, A.R., Reddy, J.S., Rao, B.V. A short, simple and general approach for the synthesis of (3S,4S)-3-methoxy-4-methylamino pyrrolidine and (3S,4R)-3-methoxy-4-methylamino pyrrolidine. Tetrahedron Lett 2003, 44(30): 5687-9. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (XXXVIII) | 69233 | (3S,4S)-tert-butyl 3-((tert-butoxycarbonyl)amino)-4-methoxypyrrolidine-1-carboxylate | C15H28N2O5 | 详情 | 详情 | |
| (XLIX) | 69242 | (R)-1-((S)-2,2-dimethyl-1,3-dioxolan-4-yl)ethane-1,2-diol | C7H14O4 | 详情 | 详情 | |
| (L) | 69243 | (R)-2-((S)-2,2-dimethyl-1,3-dioxolan-4-yl)-2-hydroxyethyl 4-methylbenzenesulfonate | C14H20O6S | 详情 | 详情 | |
| (LI) | 69244 | (R)-2-azido-1-((S)-2,2-dimethyl-1,3-dioxolan-4-yl)ethanol | C7H13N3O3 | 详情 | 详情 | |
| (LII) | 69245 | (R)-4-((S)-2-azido-1-methoxyethyl)-2,2-dimethyl-1,3-dioxolane | C8H15N3O3 | 详情 | 详情 | |
| (LIII) | 69246 | (2S,3R)-4-azido-3-methoxybutane-1,2-diol | C5H11N3O3 | 详情 | 详情 | |
| (LIV) | 69247 | (2S,3R)-4-azido-3-methoxybutane-1,2-diyl bis(4-methylbenzenesulfonate) | C19H23N3O7S2 | 详情 | 详情 | |
| (LV) | 69248 | (3S,4R)-4-methoxypyrrolidin-3-yl 4-methylbenzenesulfonate | C12H17NO4S | 详情 | 详情 | |
| (LVI) | 69249 | (3S,4R)-tert-butyl 3-methoxy-4-(tosyloxy)pyrrolidine-1-carboxylate | C17H25NO6S | 详情 | 详情 | |
| (LVII) | 69250 | (3S,4S)-tert-butyl 3-azido-4-methoxypyrrolidine-1-carboxylate | 429673-78-9 | C10H18N4O3 | 详情 | 详情 |
| (LVIII) | 69251 | trans-3-Amino-4-methoxy-pyrrolidine-1-carboxylic acid tert-butyl ester;(3S,4S)-tert-butyl 3-amino-4-methoxypyrrolidine-1-carboxylate | 429673-79-0 | C10H20N2O3 | 详情 | 详情 |
合成路线6
The reaction of 2,6-dichloropyridine (I) with BuLi and CO2 in THF gives 2,6-dichloropyridine-3-carboxylic acid (II) (1), which by reaction with refluxing SOCl2 yields the acyl chloride (III). The reaction of (III) with malonic ester by means of magnesium ethoxide in ethyl ether affords the nicotinoylacetic ester (IV), which is condensed with thiazol-2-amine (V) and triethylorthoformate by means of acetic anhydride providing the nicotinoyl acrylate (VI). The cyclization of (VI) by means of K2CO3 in hot dioxane gives the 7-chloro-4-oxo-1-(2-thiazolyl)-1,4-dihydro-1,8-naphthyridine-3-carboxylic acid ethyl ester (VII), which is condensed with the chiral pyrrolidine (VIII) by means of triethylamine yielding the proteted intermediate (IX). Finally, this compound is deprotected and hydrolyzed with hot aqueous HCl.
| 【1】 Tomita, K.; et al.; Synthesis and antitumor activity of novel 7-substituted 1,4-dihydro-4-oxo-1-(2-thiazolyl)-1,8-naphthyridine-3-carboxylic acids. 217th ACS Natl Meet (March 21 1999, Anaheim) 1999, Abst MEDI 249. |
| 【2】 Tomita, K.; Chiba, K.; Kashimoto, S.; Shibamori, K.; Tsuzuki, Y. (Dainippon Pharmaceutical Co., Ltd.); Novel cpd., process for producing the same, and antitumor agent. EP 0787726; US 5817669; WO 9534559 . |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| 16829 | Diethyl malonate | 105-53-3 | C7H12O4 | 详情 | 详情 | |
| (I) | 13573 | 2,6-Dichloropyridine | 2402-78-0 | C5H3Cl2N | 详情 | 详情 |
| (II) | 26644 | 2,6-dichloronicotinic acid | 38496-18-3 | C6H3Cl2NO2 | 详情 | 详情 |
| (III) | 26645 | 2,6-dichloronicotinoyl chloride | C6H2Cl3NO | 详情 | 详情 | |
| (IV) | 26646 | ethyl 3-(2,6-dichloro-3-pyridinyl)-3-oxopropanoate | C10H9Cl2NO3 | 详情 | 详情 | |
| (V) | 19795 | 2-Thiazolamine; 1,3-thiazol-2-amine; 1,3-thiazol-2-ylamine | 96-50-4 | C3H4N2S | 详情 | 详情 |
| (VI) | 26647 | ethyl (Z)-2-[(2,6-dichloro-3-pyridinyl)carbonyl]-3-(1,3-thiazol-2-ylamino)-2-propenoate | C14H11Cl2N3O3S | 详情 | 详情 | |
| (VII) | 26648 | ethyl 7-chloro-4-oxo-1-(1,3-thiazol-2-yl)-1,4-dihydro[1,8]naphthyridine-3-carboxylate | C14H10ClN3O3S | 详情 | 详情 | |
| (VIII) | 26649 | tert-butyl (3S,4S)-4-methoxypyrrolidinyl(methyl)carbamate | C11H22N2O3 | 详情 | 详情 | |
| (IX) | 26650 | ethyl 7-[(3S,4S)-3-[(tert-butoxycarbonyl)(methyl)amino]-4-methoxypyrrolidinyl]-4-oxo-1-(1,3-thiazol-2-yl)-1,4-dihydro[1,8]naphthyridine-3-carboxylate | C25H31N5O6S | 详情 | 详情 |
合成路线7
The chiral intermediate, the pyrrolidine (VIII) has been obtained as follows: The optical resolution of (trans)-3-amino-1-benzyl-4-methoxypyrroloidine (rac)-(X) with D-tartaric acid gives the enathiomer (S,S)(XI), which is treated with tert-butoxycarbonyl anhydride in methanol to yield the carbamate (XII). The reduction of (XII) with LiAlH4 in THF followed by reprotection with tert-butoxycarbonyl anhydride in dichloromethane affords (S,S)-1-benzyl-3-[N-(tert-butoxycarbonyl)-N-methylamino]-4-methoxypyrrolidine (XIII), which is finally debenzylated to the target compound (VIII) by hydrogenation with H2 over Pd/C in ethanol.
| 【1】 Tomita, K.; Chiba, K.; Kashimoto, S.; Shibamori, K.; Tsuzuki, Y. (Dainippon Pharmaceutical Co., Ltd.); Novel cpd., process for producing the same, and antitumor agent. EP 0787726; US 5817669; WO 9534559 . |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (VIII) | 26649 | tert-butyl (3S,4S)-4-methoxypyrrolidinyl(methyl)carbamate | C11H22N2O3 | 详情 | 详情 | |
| (X) | 26651 | (3S,4S)-1-benzyl-4-methoxy-3-pyrrolidinamine | C12H18N2O | 详情 | 详情 | |
| (XI) | 26651 | (3S,4S)-1-benzyl-4-methoxy-3-pyrrolidinamine | C12H18N2O | 详情 | 详情 | |
| (XII) | 26652 | tert-butyl (3S,4S)-1-benzyl-4-methoxypyrrolidinylcarbamate | C17H26N2O3 | 详情 | 详情 | |
| (XIII) | 26653 | tert-butyl (3S,4S)-1-benzyl-4-methoxypyrrolidinyl(methyl)carbamate | C18H28N2O3 | 详情 | 详情 |