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【结 构 式】 
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【分子编号】32407 【品名】methyl 2-(bromomethyl)acrylate 【CA登记号】 |
【 分 子 式 】C5H7BrO2 【 分 子 量 】179.01338 【元素组成】C 33.55% H 3.94% Br 44.64% O 17.88% |
合成路线1
该中间体在本合成路线中的序号：(I)The condensation of 2-(bromomethyl)acrylic acid methyl ester (I) with 1H-tetrazole (II) by means of K2CO3 in DMF gives 2-(1-tetrazolylmethyl)acrylic acid methyl ester (III), which is cyclized with 3-cyanobenzaldehyde oxime (IV) by means of NaOCl yielding the oxazoline carboxylic ester (V). The hydrolysis of (V) with LiOH in THF affords the free carboxylic acid (VI), which is condensed with 2-(6-aminopyridin-3-yl)-N-tert-butybenzenesulfonamide (VII) by means of SOCl2 and triethylamine giving the carboxamide (VIII). Finally, this compound is treated with HCl and then with ammonium acetate to eliminate the tert-butyl protecting group and to hydrolyze the cyano group to the target amidino compound.
| 【1】 Alexander, R.S.; Wesler, R.R.; Ellis, C.D.; Quan, M.L.; Liauw, A.Y.; Wong, P.C.; Lam, G.; Knabb, R.M.; Wright, M.R.; Design and synthesis of isoxazoline derivatives as factor Xa inhibitors. 2(1). J Med Chem 1999, 42, 15, 2760. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 32407 | methyl 2-(bromomethyl)acrylate | C5H7BrO2 | 详情 | 详情 | |
| (II) | 32408 | 1H-1,2,3,4-tetraazole; Tetrazole | 288-94-8 | CH2N4 | 详情 | 详情 |
| (III) | 32409 | methyl 2-(1H-1,2,3,4-tetraazol-1-ylmethyl)acrylate | C6H8N4O2 | 详情 | 详情 | |
| (IV) | 23357 | 3-[(hydroxyimino)methyl]benzonitrile | C8H6N2O | 详情 | 详情 | |
| (V) | 32410 | methyl 3-(3-cyanophenyl)-5-(1H-1,2,3,4-tetraazol-1-ylmethyl)-4,5-dihydro-5-isoxazolecarboxylate | C14H12N6O3 | 详情 | 详情 | |
| (VI) | 32411 | 3-(3-cyanophenyl)-5-(1H-1,2,3,4-tetraazol-1-ylmethyl)-4,5-dihydro-5-isoxazolecarboxylic acid | C13H10N6O3 | 详情 | 详情 | |
| (VII) | 32412 | 2-(6-amino-3-pyridinyl)-N-(tert-butyl)benzenesulfonamide | C15H19N3O2S | 详情 | 详情 | |
| (VIII) | 32413 | N-(5-[2-[(tert-butylamino)sulfonyl]phenyl]-2-pyridinyl)-3-(3-cyanophenyl)-5-(1H-1,2,3,4-tetraazol-1-ylmethyl)-4,5-dihydro-5-isoxazolecarboxamide | C28H27N9O4S | 详情 | 详情 |
合成路线2
该中间体在本合成路线中的序号：(I)The condensation of 2-(bromomethyl)acrylic acid methyl ester (I) with 1H-tetrazole (II) by means of K2CO3 in DMF gives 2-(1-tetrazolylmethyl)acrylic acid methyl ester (III), which is cyclized with 3-cyanobenzaldehyde oxime (IV) by means of NaOCl yielding the oxazoline carboxylic ester (V). The hydrolysis of (V) with LiOH in THF affords the free carboxylic acid (VI), which is condensed with 4'-amino-N-tert-butylbiphenyl-2-sulfonamide (VII) by means of SOCl2 and triethylamine giving the carboxamide (VIII). Finally, this compound is treated with HCl and then with ammonium acetate to eliminate the tert-butyl protecting group and to hydrolyze the cyano group to the target amidino compound.
| 【1】 Alexander, R.S.; Wesler, R.R.; Ellis, C.D.; Quan, M.L.; Liauw, A.Y.; Wong, P.C.; Lam, G.; Knabb, R.M.; Wright, M.R.; Design and synthesis of isoxazoline derivatives as factor Xa inhibitors. 2(1). J Med Chem 1999, 42, 15, 2760. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 32407 | methyl 2-(bromomethyl)acrylate | C5H7BrO2 | 详情 | 详情 | |
| (II) | 32408 | 1H-1,2,3,4-tetraazole; Tetrazole | 288-94-8 | CH2N4 | 详情 | 详情 |
| (III) | 32409 | methyl 2-(1H-1,2,3,4-tetraazol-1-ylmethyl)acrylate | C6H8N4O2 | 详情 | 详情 | |
| (IV) | 23357 | 3-[(hydroxyimino)methyl]benzonitrile | C8H6N2O | 详情 | 详情 | |
| (V) | 32410 | methyl 3-(3-cyanophenyl)-5-(1H-1,2,3,4-tetraazol-1-ylmethyl)-4,5-dihydro-5-isoxazolecarboxylate | C14H12N6O3 | 详情 | 详情 | |
| (VI) | 32411 | 3-(3-cyanophenyl)-5-(1H-1,2,3,4-tetraazol-1-ylmethyl)-4,5-dihydro-5-isoxazolecarboxylic acid | C13H10N6O3 | 详情 | 详情 | |
| (VII) | 23363 | 4'-amino-N-(tert-butyl)[1,1'-biphenyl]-2-sulfonamide | C16H20N2O2S | 详情 | 详情 | |
| (VIII) | 32414 | N-[2'-[(tert-butylamino)sulfonyl][1,1'-biphenyl]-4-yl]-3-(3-cyanophenyl)-5-(1H-1,2,3,4-tetraazol-1-ylmethyl)-4,5-dihydro-5-isoxazolecarboxamide | C29H28N8O4S | 详情 | 详情 |
合成路线3
该中间体在本合成路线中的序号：(I)Conversion of the bromo derivative (I) into the corresponding azido compound (II) is performed by treatment with NaN3 in DMSO. Separately, aldehyde (III) is treated with hydroxylamine to provide oxime (IV), which is then condensed with derivative (II) by means of bleach in CH2Cl2 to furnish isoxazoline derivative (V). Hydrolysis of the methyl ester of (V) by means of LiOH in THF affords carboxylic acid (VI), which is treated with SOCl2 and then condensed with amine (VII) by means of Et3N to give carboxamide (VIII). Reduction of the azido moiety of (VIII) by means of P(OEt)3 in THF yields amine (IX), which is then derivatized by reaction with mesyl chloride (MeSO2Cl) to provide mesylate (X). Finally, the desired compound is obtained by treatment of (X) with TFA for tert-butyl group removal, followed by reduction of the nitro moiety with SnCl2.
| 【1】 Wong, P.C.; Ellis, C.D.; Rossi, K.A.; Wexler, R.R.; Wright, M.R.; Quan, M.L.; Lam, P.Y.; He, M.Y.; Knabb, R.M.; Liauw, A.Y.; Nonbenzamidine isoxazolin derivatives as factor Xa inhibitors. 221st ACS Natl Meet (April 1 2001, San Diego) 2001, Abst MEDI 52. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 32407 | methyl 2-(bromomethyl)acrylate | C5H7BrO2 | 详情 | 详情 | |
| (II) | 50083 | methyl 2-(azidomethyl)acrylate | C5H7N3O2 | 详情 | 详情 | |
| (III) | 50084 | 3-Nitro-4-chlorobenzaldehyde; 4-Chloro-3-nitrobenzaldehyde | 16588-34-4 | C7H4ClNO3 | 详情 | 详情 |
| (IV) | 50085 | N-(4-chloro-3-nitrobenzyl)hydroxylamine; 1-chloro-4-[(hydroxyamino)methyl]-2-nitrobenzene | C7H7ClN2O3 | 详情 | 详情 | |
| (V) | 50086 | methyl 5-(azidomethyl)-3-(4-chloro-3-nitrophenyl)-4,5-dihydro-5-isoxazolecarboxylate | C12H10ClN5O5 | 详情 | 详情 | |
| (VI) | 50087 | 5-(azidomethyl)-3-(4-chloro-3-nitrophenyl)-4,5-dihydro-5-isoxazolecarboxylic acid | C11H8ClN5O5 | 详情 | 详情 | |
| (VII) | 32412 | 2-(6-amino-3-pyridinyl)-N-(tert-butyl)benzenesulfonamide | C15H19N3O2S | 详情 | 详情 | |
| (VIII) | 50088 | 5-(azidomethyl)-N-(5-[2-[(tert-butylamino)sulfonyl]phenyl]-2-pyridinyl)-3-(4-chloro-3-nitrophenyl)-4,5-dihydro-5-isoxazolecarboxamide | C26H25ClN8O6S | 详情 | 详情 | |
| (IX) | 50089 | 5-(aminomethyl)-N-(5-[2-[(tert-butylamino)sulfonyl]phenyl]-2-pyridinyl)-3-(4-chloro-3-nitrophenyl)-4,5-dihydro-5-isoxazolecarboxamide | C26H27ClN6O6S | 详情 | 详情 | |
| (X) | 50090 | N-(5-[2-[(tert-butylamino)sulfonyl]phenyl]-2-pyridinyl)-3-(4-chloro-3-nitrophenyl)-5-[[(methylsulfonyl)amino]methyl]-4,5-dihydro-5-isoxazolecarboxamide | C27H29ClN6O8S2 | 详情 | 详情 |
合成路线4
该中间体在本合成路线中的序号：(VI)Mesylate (II), prepared from the (hydroxymethyl)phosphinic acid derivative (I), was condensed with the protected glucosamine (III) in the presence of NaH to produce the phosphinate adduct (IV) as a diastereomeric mixture. Selective removal of the P-acetyl ketal group of (IV) with chlorotrimethylsilane and a trace of water gave rise to the deprotected phosphinate (V). This was subjected to Arbuzov reaction with methyl (bromomethyl)acrylate (VI) under silylating conditions to afford the substituted phosphinate (VII). Concomitant double-bond hydrogenation and hydrogenolysis of the benzyl and benzylidene groups of (VII) was effected by hydrogenation over Pd/C. The resulting product (VIII) was then peracetylated with acetic anhydride/pyridine to give the triacetate (IX). Treatment of (IX) with aminoethanol in THF selectively removed the anomeric acetate to produce (X) as a complex diastereomeric mixture.
| 【1】 Reck, F.; et al.; Inhibitors of the bacterial cell wall biosynthesis enzyme MurC. Bioorg Med Chem Lett 2001, 11, 11, 1451. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 53154 | ethyl 1,1-diethoxyethyl(hydroxymethyl)phosphinate | n/a | C9H21O5P | 详情 | 详情 |
| (II) | 53155 | [(1,1-diethoxyethyl)(ethoxy)phosphoryl]methyl methanesulfonate | n/a | C10H23O7PS | 详情 | 详情 |
| (III) | 53156 | N-[(2R,4aR,6S,7R,8R,8aS)-6-(benzyloxy)-8-hydroxy-2-phenylhexahydropyrano[3,2-d][1,3]dioxin-7-yl]acetamide | n/a | C22H25NO6 | 详情 | 详情 |
| (IV) | 53157 | ethyl {[(2R,4aR,6S,7R,8R,8aR)-7-(acetylamino)-6-(benzyloxy)-2-phenylhexahydropyrano[3,2-d][1,3]dioxin-8-yl]oxy}methyl(1,1-diethoxyethyl)phosphinate | n/a | C31H44NO10P | 详情 | 详情 |
| (V) | 53158 | ethyl {[(2R,4aR,6S,7R,8R,8aR)-7-(acetylamino)-6-(benzyloxy)-2-phenylhexahydropyrano[3,2-d][1,3]dioxin-8-yl]oxy}methylphosphinate | n/a | C25H32NO8P | 详情 | 详情 |
| (VI) | 32407 | methyl 2-(bromomethyl)acrylate | C5H7BrO2 | 详情 | 详情 | |
| (VII) | 53159 | methyl 2-{[({[(2R,4aR,6S,7R,8R,8aR)-7-(acetylamino)-6-(benzyloxy)-2-phenylhexahydropyrano[3,2-d][1,3]dioxin-8-yl]oxy}methyl)(ethoxy)phosphoryl]methyl}acrylate | n/a | C30H38NO10P | 详情 | 详情 |
| (VIII) | 53160 | methyl 3-[({[(2S,3R,4R,5R,6R)-3-(acetylamino)-2,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}methyl)(ethoxy)phosphoryl]-2-methylpropanoate | n/a | C16H30NO10P | 详情 | 详情 |
| (IX) | 53161 | methyl 3-[[({(2R,3R,4R,5R,6R)-3-(acetylamino)-2,5-bis(acetyloxy)-6-[(acetyloxy)methyl]tetrahydro-2H-pyran-4-yl}oxy)methyl](ethoxy)phosphoryl]-2-methylpropanoate | n/a | C22H36NO13P | 详情 | 详情 |
| (X) | 53162 | methyl 3-[[({(3R,4R,5R,6R)-3-(acetylamino)-5-(acetyloxy)-6-[(acetyloxy)methyl]-2-hydroxytetrahydro-2H-pyran-4-yl}oxy)methyl](ethoxy)phosphoryl]-2-methylpropanoate | n/a | C20H34NO12P | 详情 | 详情 |