合成路线1

Previously, it was reported that D-ribose was synthesized from D-xylose, the epimer of D-ribose, via a stereoselective oxidation-reduction procedure, albeit in low yield. This strategy was adapted to prepare the L-ribose derivative (V) for the practical synthesis of L-FMAU as well as related nucleosides. After pyridinium dichromate (PDC) oxidation, ketone (III) was reduced to an alcohol using NaBH4. It should be mentioned that due to the stereoelectronic effects of the 1,2-O-isopropylidene group, the hydride preferentially attacks from the beta-face. After a series of reactions, compound (V) was obtained and treated with saturated hydrogen chloride in CH2Cl2 at 0 C followed by the migration of the benzoyl group and treatment with SO2Cl2 and imidazole in DMF/CH2Cl2 to give a imidazolyl intermediate. The imidazole derivative was fluorinated with a mixture of KHF2 and 48% HF/H2O to give 1,3,5-tri-O-benzoyl-2-fluoro-alpha-L-arabinofuranose, which was converted to bromosugar (VI) using 45% HBr/AcOH. Without further purification, the bromosugar was condensed with silylated thymine in anhydrous CHCl3 in refluxing conditions to give protected L-FMAU with high stereoselectivity, which was further treated with NH3/CH3OH for debenzoylation to give L-FMAU. Due to the availability and high cost of L-xylose, however, we have developed another procedure from L-arabinose. L-Arabinose is a natural sugar with reasonable cost. The anomeric position was protected with a benzyl group in acidic conditions to the pyranose form and the vicinal cis-hydroxy groups were protected with an isopropylidene group. In similar conditions as for L-xylose, PDC was used for the oxidation reaction to obtain ketone (IV), which was reduced with NaBH4 in methanol with high stereoselectivity as described for L-xylose. After deprotection of compound (IV) using 4% CF3CO2H, the resulting L-ribose was sequentially treated with 1% HCl/MeOH, BzCl/pyridine and H2SO4/AcO/AcOH to obtain compound (V). The remaining steps to L-FMAU were the same as described for the L-xylose route.