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【结 构 式】 
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【分子编号】43458 【品名】(3S)-4-(benzyloxy)-3-[[(benzyloxy)carbonyl]amino]-4-oxobutyric acid 【CA登记号】4779-31-1 |
【 分 子 式 】C19H19NO6 【 分 子 量 】357.363 【元素组成】C 63.86% H 5.36% N 3.92% O 26.86% |
合成路线1
该中间体在本合成路线中的序号：(VII)Amine intermediate (XIV) has been obtained as follows: N-(Carbobenzoxy)-L-aspartic acid alpha-benzyl ester (VII) was converted to acid chloride (VIII) and then coupled to 2-aminophenyl disulfide (IX) to afford the bis-amide (X). Reductive cleavage of the disulfide group of (X), followed by basic cyclization, produced the benzothiazole (XI) (2). Hydrolysis of the benzyl ester group of (XI) and subsequent coupling of the resulting carboxylic acid (XII) with piperidine (VI) gave amide (XIII). The carbobenzoxy group of (XII) was then cleaved with HBr in HOAc, yielding intermediate amine (XIV).
| 【1】 Baettig, U.; et al.; The design and synthesis of thrombin inhibitors: Analogues of MD805 containing non-polar surrogates for arginine at the P1 position. Bioorg Med Chem Lett 2000, 10, 14, 1563. |
| 【2】 Smith, G.P.; Brundish, D.E.; Menear, K.A.; Walker, C.V.; Herold, P.; Fullerton, J.D.; Allen, M.C.; Le Grand, D.M.; Kane, P.D.; Cockcroft, X.-L.; Wathey, W.B.; Butler, P.I.; Hatto, J.D.I.; Hayler, J.D. (Novartis AG); Tetrahydroquinoline inhibitors of trypsin and thrombin. GB 2312674 . |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (VI) | 28517 | 4-(2-fluoroethyl)piperidine | C7H14FN | 详情 | 详情 | |
| (VII) | 43458 | (3S)-4-(benzyloxy)-3-[[(benzyloxy)carbonyl]amino]-4-oxobutyric acid | 4779-31-1 | C19H19NO6 | 详情 | 详情 |
| (VIII) | 43459 | benzyl (2S)-2-[[(benzyloxy)carbonyl]amino]-4-chloro-4-oxobutanoate | C19H18ClNO5 | 详情 | 详情 | |
| (IX) | 43460 | 2-[(2-aminophenyl)disulfanyl]aniline; 2-[(2-aminophenyl)disulfanyl]phenylamine | 1141-88-4 | C12H12N2S2 | 详情 | 详情 |
| (X) | 43461 | benzyl (2S)-4-[2-([2-[((3S)-4-(benzyloxy)-3-[[(benzyloxy)carbonyl]amino]-4-oxobutanoyl)amino]phenyl]disulfanyl)anilino]-2-[[(benzyloxy)carbonyl]amino]-4-oxobutanoate | C50H46N4O10S2 | 详情 | 详情 | |
| (XI) | 43462 | benzyl (2S)-3-(1,3-benzothiazol-2-yl)-2-[[(benzyloxy)carbonyl]amino]propanoate | C25H22N2O4S | 详情 | 详情 | |
| (XII) | 43463 | (2S)-3-(1,3-benzothiazol-2-yl)-2-[[(benzyloxy)carbonyl]amino]propionic acid | C18H16N2O4S | 详情 | 详情 | |
| (XIII) | 43464 | benzyl (1S)-1-(1,3-benzothiazol-2-ylmethyl)-2-[4-(2-fluoroethyl)-1-piperidinyl]-2-oxoethylcarbamate | C25H28FN3O3S | 详情 | 详情 | |
| (XIV) | 43465 | (2S)-2-amino-3-(1,3-benzothiazol-2-yl)-1-[4-(2-fluoroethyl)-1-piperidinyl]-1-propanone | C17H22FN3OS | 详情 | 详情 |
合成路线2
该中间体在本合成路线中的序号：(I)Condensation between benzyl N-Cbz-alpha-glutamate (I) and tert-butyl acrylate (II) in THF in the presence of tert-BuOK followed by treatment with ammonium acetate in refluxing methanol provides pyrroline acetic acid (III), which is converted into the corresponding benzyl ester (IV) by reaction with benzyl bromide and DIEA in refluxing CH2Cl2. Protection of (IV) with Boc2O and DMAP in CH2Cl2 yields the bis-Boc derivative (V), which is subjected to hydrogenation over Pd/C in HOAc to afford a mixture diastereomers (VI) and (VI’) which are inseparable chromatographically. The mixture of (VI)/(VI') is subjected to reaction with carbamoyl chloride (VII) in CH2Cl2 in the presence of DIEA or pyridine to give the corresponding N-ethyl-N-isopropylureas from which diastereomer (2S,3R,4R)-(VIII) is chromatographically isolated. Derivative (2S,3R,4R)-(VIII) is subjected to Curtius rearrangement using diphenylphosphoryl azide (DPPA) and Et3N in toluene in the presence of benzyl alcohol to give the Cbz-protected amine (IX), which is then deprotected by hydrogenation over Pd in formic acid/MeOH to yield free amine (X). Coupling of (X) with trifluoroacetic acid (XI) by means of EDCI and HOBt in CH2Cl2 furnishes acetamide (XII), which is finally converted into the desired product by removal of the corresponding protecting groups with TFA in CH2Cl2.
| 【1】 Wang, G.T.; Chen, Y.; Wang, S.; et al.; Design, synthesis, and structural analysis of influenza neuraminidase inhibitors containing pyrrolidine cores. J Med Chem 2001, 44, 8, 1192. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (V') | 49806 | 2-[(2R,3R,4R)-3-[bis(tert-butoxycarbonyl)amino]-4-(tert-butoxycarbonyl)pyrrolidinyl]acetic acid | C21H36N2O8 | 详情 | 详情 | |
| (I) | 43458 | (3S)-4-(benzyloxy)-3-[[(benzyloxy)carbonyl]amino]-4-oxobutyric acid | 4779-31-1 | C19H19NO6 | 详情 | 详情 |
| (II) | 12760 | tert-butyl acrylate | 1663-39-4 | C7H12O2 | 详情 | 详情 |
| (III) | 49802 | 2-[3-amino-1-[(benzyloxy)carbonyl]-4-(tert-butoxycarbonyl)-2,5-dihydro-1H-pyrrol-2-yl]acetic acid | C19H24N2O6 | 详情 | 详情 | |
| (IV) | 49803 | 1-benzyl 3-(tert-butyl) 4-amino-5-[2-(benzyloxy)-2-oxoethyl]-2,5-dihydro-1H-pyrrole-1,3-dicarboxylate | C26H30N2O6 | 详情 | 详情 | |
| (V) | 49804 | 1-benzyl 3-(tert-butyl) 5-[2-(benzyloxy)-2-oxoethyl]-4-[bis(tert-butoxycarbonyl)amino]-2,5-dihydro-1H-pyrrole-1,3-dicarboxylate | C36H46N2O10 | 详情 | 详情 | |
| (V) | 49805 | 2-[(2S,3R,4R)-3-[bis(tert-butoxycarbonyl)amino]-4-(tert-butoxycarbonyl)pyrrolidinyl]acetic acid | C21H36N2O8 | 详情 | 详情 | |
| (VII) | 49807 | 2-[(chlorocarbonyl)(ethyl)amino]propane | C6H12ClNO | 详情 | 详情 | |
| (VIII) | 49808 | 2-((2S,3R,4R)-3-[bis(tert-butoxycarbonyl)amino]-4-(tert-butoxycarbonyl)-1-[[ethyl(isopropyl)amino]carbonyl]pyrrolidinyl)acetic acid | C27H47N3O9 | 详情 | 详情 | |
| (IX) | 49809 | tert-butyl (3R,4R,5S)-5-([[(benzyloxy)carbonyl]amino]methyl)-4-[bis(tert-butoxycarbonyl)amino]-1-[[ethyl(isopropyl)amino]carbonyl]-3-pyrrolidinecarboxylate | C34H54N4O9 | 详情 | 详情 | |
| (X) | 49810 | tert-butyl (3R,4R,5S)-5-(aminomethyl)-4-[bis(tert-butoxycarbonyl)amino]-1-[[ethyl(isopropyl)amino]carbonyl]-3-pyrrolidinecarboxylate | C26H48N4O7 | 详情 | 详情 | |
| (XI) | 49811 | trifluoro-lambda(5)-azanecarboxylic acid | CH2F3NO2 | 详情 | 详情 | |
| (XII) | 49812 | tert-butyl (3R,4R,5S)-4-[bis(tert-butoxycarbonyl)amino]-1-[[ethyl(isopropyl)amino]carbonyl]-5-([[(trifluoro-lambda(5)-azanyl)carbonyl]amino]methyl)-3-pyrrolidinecarboxylate | C27H48F3N5O8 | 详情 | 详情 |