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【结 构 式】

【分子编号】39993

【品名】[(2S,3S,4S,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-4-hydroxytetrahydro-2-furanyl]methyl benzoate

【CA登记号】

【 分 子 式 】C23H20N2O8

【 分 子 量 】452.42048

【元素组成】C 61.06% H 4.46% N 6.19% O 28.29%

与该中间体有关的原料药合成路线共 2 条

合成路线1

该中间体在本合成路线中的序号:(V)

Uracil (I) was silylated with hexamethyldisilazan, and the resulting bis-silylated uracil (II) was condensed with protected xylofuranose (III) in the presence of trimethylsilyl triflate to afford nucleoside (IV). Selective hydrolysis of the acetate ester of (IV) employing hydrazine and AcOH in pyridine yielded (V). Isomerization of xylofuranosyl uracil (V) to the arabinofuranosyl analogue (VI) was achieved by reaction with dicyclohexylcarbodiimide and dichloroacetic acid followed by treatment with NaBH4. Subsequent deoxygenation of the 2'-hydroxyl group of (VI) was effected via condensation with phenyl chlorothionoformate, and then reduction of the resulting thiocarbonate (VII) with tris(trimethylsilyl)silane and azobis(isobutyronitrile) (AIBN). The resultant 2'-deoxyuridine derivative (VIII) was treated with Lawesson's reagent to give the thiocarbonyl analogue (IX). Finally, sulfur displacement with concomitant benzoate ester hydrolysis employing methanolic ammonia at 100 C in a pressure bomb furnished the title compound.

1 Bryant, M.L.; Gosselin, G.; Imbach, J.-L. (CNRS (Centre National de la Recherche Scientifique); Novirio Pharmaceuticals Ltd.); beta-L-2'-Deoxy-nucleosides for the treatment of hepatitis B. WO 0009531 .
中间体序号 中间体编号 品名 CAS号 分子式 供应商 用于合成
(I) 30921 2,4(1H,3H)-pyrimidinedione; Uracil 66-22-8 C4H4N2O2 详情 详情
(II) 29619 2,4-bis[(trimethylsilyl)oxy]pyrimidine; trimethylsilyl 2-[(trimethylsilyl)oxy]-4-pyrimidinyl ether C10H20N2O2Si2 详情 详情
(III) 39991 [(2S,3R,4S)-4,5-bis(acetoxy)-3-(benzoyloxy)tetrahydro-2-furanyl]methyl benzoate C23H22O9 详情 详情
(IV) 39992 (2S,3R,4S,5S)-4-(acetoxy)-2-[(benzoyloxy)methyl]-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-3-furanyl benzoate C25H22N2O9 详情 详情
(V) 39993 [(2S,3S,4S,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-4-hydroxytetrahydro-2-furanyl]methyl benzoate C23H20N2O8 详情 详情
(VI) 39994 [(2S,3R,4R,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-4-hydroxytetrahydro-2-furanyl]methyl benzoate C23H20N2O8 详情 详情
(VII) 39995 [(2S,3S,4R,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-4-[(phenoxycarbothioyl)oxy]tetrahydro-2-furanyl]methyl benzoate C30H24N2O9S 详情 详情
(VIII) 39996 [(2S,3R,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-2-furanyl]methyl benzoate C23H20N2O7 详情 详情
(IX) 40005 [(2S,3R,5S)-3-(benzoyloxy)-5-[2-oxo-4-thioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-2-furanyl]methyl benzoate C23H20N2O6S 详情 详情

合成路线2

该中间体在本合成路线中的序号:(XXX)

Reaction of L-xylose (XXII) with acetone and H2SO4 gives the acetonide (XXIII), which by acylation with benzoyl chloride in pyridine/chloroform yields the dibenzoate (XXIV). The hydrolysis of acetonide (XXIV) with acetic acid to the dihydroxy sugar (XXV) followed by acylation with acetic anhydride provides the tetracylated L-xylose (XXVI). Condensation of compound (XXVI) with 2,4-bis(trimethylsilyloxy)pyrimidine (XXVII) - obtained by silylation of uracil (XXVIII) with hexamethyldisilazane - by means of trimethylsilyl triflate in 1,2-dichloroethane affords nucleoside (XXIX). Selective hydrolysis of the acetate ester of compound (XXIX) using hydrazine and AcOH in pyridine yields the dibenzoylated xylofuranosyl-uracil (XXX). Isomerization of compound (XXX) to the arabinofuranosyl analogue (XXXI) is achieved by reaction with dicyclohexylcarbodiimide and dichloroacetic acid in DMSO/benzene, followed by treatment with NaBH4 in EtOH/benzene. Subsequent deoxygenation of the 2'-hy-droxyl group of compound (XXXI) to afford 2'-deoxynucleoside (XX) is effected via condensation with phenyl chlorothionoformate, and then reduction of the resulting thiocarbonate (XXXII) with tris(trimethylsilyl)silane and AIBN. Iodination of the uracil ring of (XX) with I2 and cerium ammonium nitrate (CAN) in acetonitrile produces the 5-iodo derivative (XXXIII), which is protected at the 3-nitrogen atom by condensation with p-toluoyl chloride to yield the protected nucleoside (XXXIV). Introduction of the 5-methyl group to give the thymidine derivative (XXXV) is then effected by reaction of the 5-iodouracil (XXXIV) with tetramethyltin in the presence of palladium catalyst. Finally, all protecting groups of compound (XXXV) are removed by treatment with methanolic ammonia.

1 Gosselin, G.; Bergogne, M.C.; Imbach, J.L.; Synthesis and antiviral evaluation of beta-L-xylo-furanosyl nucleosides of the five naturally occurring nucleic acid bases. J Heterocycl Chem 1993, 30, 5, 1229.
2 Sorbera, L.A.; Castañer, J.; Castañer, R.M.; Bayes, M.; Telbivudine. Drugs Fut 2003, 28, 9, 870.
3 Gosselin, G.; Imbach, J.-L.; Aubertin, A.-M.; Sommadossi, J.-P.; Schinazi, R.F. (CNRS (Centre National de la Recherche Scientifique)); 2' or 3'-Deoxy and 2'-dideoxy-beta-L-pentafuranonucleoside cpds., method of preparation and application in therapy, especially as anti-viral agents. DE 2709754; EP 0717748; WO 9507287 .
4 Bryant, M.L.; Gosselin, G.; Imbach, J.-L. (CNRS (Centre National de la Recherche Scientifique); Novirio Pharmaceuticals Ltd.); beta-L-2'-Deoxy-nucleosides for the treatment of hepatitis B. WO 0009531 .
中间体序号 中间体编号 品名 CAS号 分子式 供应商 用于合成
(XX) 39996 [(2S,3R,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-2-furanyl]methyl benzoate C23H20N2O7 详情 详情
(XXII) 62919 (3S,4S,5S)-5-(hydroxymethyl)tetrahydro-2,3,4-furantriol C5H10O5 详情 详情
(XXIII) 41907 (3aR,5R,6S,6aR)-5-(hydroxymethyl)-2,2-dimethyltetrahydrofuro[2,3-d][1,3]dioxol-6-ol 20031-21-4 C8H14O5 详情 详情
(XXIV) 56701 [(3aR,5R,6S,6aR)-6-(benzoyloxy)-2,2-dimethyltetrahydrofuro[2,3-d][1,3]dioxol-5-yl]methyl benzoate C22H22O7 详情 详情
(XXV) 56702 [(2R,3R,4R,5S)-3-(benzoyloxy)-4,5-dihydroxytetrahydro-2-furanyl]methyl benzoate C19H18O7 详情 详情
(XXVI) 39991 [(2S,3R,4S)-4,5-bis(acetoxy)-3-(benzoyloxy)tetrahydro-2-furanyl]methyl benzoate C23H22O9 详情 详情
(XXVII) 29619 2,4-bis[(trimethylsilyl)oxy]pyrimidine; trimethylsilyl 2-[(trimethylsilyl)oxy]-4-pyrimidinyl ether C10H20N2O2Si2 详情 详情
(XXVIII) 30921 2,4(1H,3H)-pyrimidinedione; Uracil 66-22-8 C4H4N2O2 详情 详情
(XXIX) 39992 (2S,3R,4S,5S)-4-(acetoxy)-2-[(benzoyloxy)methyl]-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-3-furanyl benzoate C25H22N2O9 详情 详情
(XXX) 39993 [(2S,3S,4S,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-4-hydroxytetrahydro-2-furanyl]methyl benzoate C23H20N2O8 详情 详情
(XXXI) 39994 [(2S,3R,4R,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-4-hydroxytetrahydro-2-furanyl]methyl benzoate C23H20N2O8 详情 详情
(XXXII) 39995 [(2S,3S,4R,5S)-3-(benzoyloxy)-5-[2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-4-[(phenoxycarbothioyl)oxy]tetrahydro-2-furanyl]methyl benzoate C30H24N2O9S 详情 详情
(XXXIII) 40002 [(2S,3R,5S)-3-(benzoyloxy)-5-[5-iodo-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-2-furanyl]methyl benzoate C23H19IN2O7 详情 详情
(XXXIV) 40003 [(2S,3R,5S)-3-(benzoyloxy)-5-[5-iodo-3-(4-methylbenzoyl)-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-2-furanyl]methyl benzoate C31H25IN2O8 详情 详情
(XXXV) 40004 [(2S,3R,5S)-3-(benzoyloxy)-5-[5-methyl-3-(4-methylbenzoyl)-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-2-furanyl]methyl benzoate C32H28N2O8 详情 详情
Extended Information