合成路线1

The 1alpha,3beta-diol (Ia) derived from ergosterol was used as the starting material. The alcohol (Ia) was converted to methoxymethyl derivative (Ib) by reaction with chloromethyl methyl ether (4 eq) in dichloromethane for 16 h, which was oxidized with 3 eq of N-methylmorpholine N-oxide in the presence of osmium tetroxide (0.5 eq) in tert-butanol:tetrahydrofuran:water (10:3:1) for 4 h at room temperature to afford the 22,23-diol. The resulting diol was then cleaved by sodium metaperiodate (2.5 eq) in aqueous tetrahydrofuran (5 h, room temperature) to give the 22-aldehyde (IIa) in 30% yield from (Ia). Reaction of (IIa) with 6 eq of vinyl magnesium bromide (tetrahydrofuran, 0 C to room temperature, 2 h) gave the allyl alcohol (IIb) as a mixture of C-22 diastereoisomers. The Claisen reaction of (IIb) with 10 eq of triethyl orthopropionate and a catalytic amount of propionic acid (benzene reflux, 16 h) gave gamma,delta-unsaturated ester (IIa) in 70% yield from (IIa). The lithium enolate of (IIIa) generated by lithium N-isopropylcyclohexylamide in tetrahydrofuran at -78 C was reacted with oxygen for 1 h and subsequent reduction with triethyl phosphine at -78 C gave the 25-hydroxy ester (IIIb) in 87% yield. (IIIb) was shown to be a 1:1 mixture of C-25 diastereoisomers by high-pressure liquid chromatographic (HPLC) analysis. Alkaline hydrolysis (KOH, methanol, 60 C, 2.5 h) of the ester gave the hydroxy acid (IIIc) in 87% yield. (IIIc) was treated with 6 eq of I2 in dichloromethane in the presence of pyridine (15 eq) to give the iodolactone (IVa) in 74% yield, which was separated into isomers by silica gel column chromatography in a 1.5:1 ratio.