【结 构 式】 |
【分子编号】12166 【品名】1-[(2R,4S,5R)-4-Hydroxy-5-[(trityloxy)methyl]tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione 【CA登记号】 |
【 分 子 式 】C29H28N2O5 【 分 子 量 】484.5518 【元素组成】C 71.88% H 5.82% N 5.78% O 16.51% |
合成路线1
该中间体在本合成路线中的序号:(I)1) The mesylation of 5'-O-tritylthymidine with mesyl chloride gives the corresponding 3'-O-mesyl derivative (II), which is cyclized by means of potassium phthalimide (III) in hot DMF water, yielding 5'-O-trityl-2,3'-anhydrothymidine (IV). The reaction of (IV) with sodium azide in refluxing DMF - water affords 5'-O-trityl-3'-azido-3'-deoxy thymidine (V), which is finally deprotected with 80% aqueous acetic acid at 100 C.
【1】 Glinski, R.P.; et al.; Nucleotide synthesis. IV. Phosphorylated 3'-amino-3'-deoxythymidine and 5'-amino-5'-deoxythymidine and derivatives. J Org Chem 1973, 38, 25, 4299. |
【2】 Castaner, J.; Prous, J.; BW-A 509 U. Drugs Fut 1986, 11, 12, 1017. |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(I) | 12166 | 1-[(2R,4S,5R)-4-Hydroxy-5-[(trityloxy)methyl]tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione | C29H28N2O5 | 详情 | 详情 | |
(II) | 12196 | (2R,3S,5R)-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-2-[(trityloxy)methyl]tetrahydro-3-furanyl methanesulfonate | C30H30N2O7S | 详情 | 详情 | |
(III) | 10926 | (1,3-Dioxo-1,3-dihydro-2H-isoindol-2-yl)potassium | C8H4KNO2 | 详情 | 详情 | |
(IV) | 12168 | (1R,9R,10R)-4-Methyl-10-[(trityloxy)methyl]-8,11-dioxa-2,6-diazatricyclo[7.2.1.0(2,7)]dodeca-3,6-dien-5-one | C29H26N2O4 | 详情 | 详情 | |
(V) | 24469 | 1-[4-azido-5-[(trityloxy)methyl]tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione | C29H27N5O4 | 详情 | 详情 |
合成路线2
该中间体在本合成路线中的序号:(VI)Alovudine can be obtained by several related ways: 1) The acylation of thymidine (I) with 4-chlorobenzoylchloride (II) in pyridine gives 5'-O-(4-chlorobenzoyl)thymidine (IX), which is then treated with diethylamino sulfur trifluoride in methylene chloride at -79 C, and finally with NaHCO3 in refluxing methanol. 2) By reaction of 2,3'-anhydro-1-(2'-deoxy-beta-D-xylofuranosyl)thymine (III) with AlF3 and 1% HF in anhydrous dioxan at 150-7 C. 3) By reaction of (III) with KHF2 or NH4F in diethylene glycol at 190C, or in ethanol at 150 C. 4) The reaction of 2,3'-anhydro-5'-O-(methylsufonyl)-1-(2'-deoxy-beta-D-xylofuranosyl) thymine (IV) with AlF3 and HF as before gives 3'-deoxy-3'-fluoro-5'-O-(methylsulfonyl)thymidine (V), which is then treated with NaOH in refluxing ethanol. 5) By reaction of 5'-O-(triphenylmethyl)thymidine (VI) with diethylamino sulfur trifluoride in THF, and then with aqueous, NaHCO3. 6) By reaction of 3'-O-(methylsulfonyl)thymidine (VII) with KHF2 or NH4F in diethylene glycol at 190 C. 7) By reaction of 2,3'-anhydro-5'-O-(triphenylmethyl)-1-(2'-deoxy-beta-D-xylofuranosyl) thymine (VIII) with 4-6% HF in anhydrous dioxan at 90 C.
【1】 Selway, J.W.T.; Beacham III, L.M.; Daluge, S.M.; Tuttle, J.V.; Krenitsky, T.A. (Glaxo Wellcome plc); 2,3-Dideoxy-3'-fluorothymidine and related cpds. for the treatment of adenovirus infections. AU 8821429; EP 0305117; EP 0479336; JP 1989068325; US 5070078 . |
【2】 Etzold, G.; Hintsche, R.; Langen, P. (Akademie der Wissenschaften der DDR); Cytostatically-active 2',3'-didesoxy-3'-fluoropyrimidine nucleosides. GB 1189973 . |
【3】 Castaner, J.; Hoshi, A.; Alovudine. Drugs Fut 1994, 19, 3, 221. |
【4】 Baba, M.; De Clercq, E.; Pauwels, R.; Herdewijn, P.; Balzarini, J.; Vanderhaeghe, H.; Broder, S.; 3'-Substituted 2',3'-dideoxynucleoside analogues as potential anti-HIV (HTLV-III/LAV) agents. J Med Chem 1987, 30, 8, 1270-8. |
【5】 Kowollik, G.; Etzold, G.; Hintsche, R.; Langen, P.; Synthesis and reactivity of 3'-fluoro and 3'-chloro-3'-desoxy-thymidine. Tetrahedron 1971, 27, 2463-72. |
【6】 Langen, P.; Von Janta-Lipinski, M.; Gaertner, K.; Etzold, G.; Von Kowollik, G.; A novel access to 1-(2,3-didesoxy-3-fluoro-beta-D-ribofuranosyl)-pyrimidines. J Prakt Chem 1973, 315, 5, 895-900. |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(I) | 12161 | Thymidine; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione | 50-89-5 | C10H14N2O5 | 详情 | 详情 |
(II) | 10295 | p-Chlorobenzoyl chloride; 4-Chlorobenzoyl chloride | 122-01-0 | C7H4Cl2O | 详情 | 详情 |
(III) | 12163 | (1R,9R,10R)-10-(Hydroxymethyl)-4-methyl-8,11-dioxa-2,6-diazatricyclo[7.2.1.0(2,7)]dodeca-3,6-dien-5-one | C10H12N2O4 | 详情 | 详情 | |
(IV) | 12164 | [(1R,9R,10R)-4-methyl-5-oxo-8,11-dioxa-2,6-diazatricyclo[7.2.1.0(2,7)]dodeca-3,6-dien-10-yl]methyl methanesulfonate | C11H14N2O6S | 详情 | 详情 | |
(V) | 12165 | [(2R,3S,5R)-3-fluoro-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-2-furanyl]methyl methanesulfonate | C11H15FN2O6S | 详情 | 详情 | |
(VI) | 12166 | 1-[(2R,4S,5R)-4-Hydroxy-5-[(trityloxy)methyl]tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione | C29H28N2O5 | 详情 | 详情 | |
(VII) | 12167 | (2R,3S,5R)-2-(hydroxymethyl)-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-3-furanyl methanesulfonate | C11H16N2O7S | 详情 | 详情 | |
(VIII) | 12168 | (1R,9R,10R)-4-Methyl-10-[(trityloxy)methyl]-8,11-dioxa-2,6-diazatricyclo[7.2.1.0(2,7)]dodeca-3,6-dien-5-one | C29H26N2O4 | 详情 | 详情 | |
(IX) | 63321 | methyl 4-[2-(tert-butylamino)acetyl]-7-hydroxy-1H-indole-2-carboxylate | C16H20N2O4 | 详情 | 详情 |
合成路线3
该中间体在本合成路线中的序号:(IVa)1) The first method ever reported involves the mesylation of thymidine (I) to give dimesylate (II), which is treated with sodium hydroxide in ethanol to yield oxetane (III). Finally, (III) is converted to stavudine by means of potassium tert-butoxide in DMSO. This process has been modified in order to obtain large quantities or to obtain [2-14C]-stavudine starting from [2-14C]-thymidine. 2) A second closely related method, but using a different protecting group strategy, involves tritylation of the primary hydroxyl group of thymidine to give (IVa), which is then mesylated to give (Va). Elimination with TBAF/THF or t-BuOK/DMSO yields compound (VI), which is finally deprotected with acetic acid. 3) A third synthesis starting from thymidine involves its protection with monomethoxytrityl chloride or picolyl chloride to give (IVb) or (IVc), respectively, which are mesylated to give (Vb) or (Vc). These are treated with phenyl diselenide and lithium aluminum hydride in tetrahydrofuran to yield compounds (VIIb) or (VIIc), which are treated with m-chloroperbenzoic acid in dichloromethane to afford (VIIIb) or (VIIIc). Finally, (VIIIb) is deprotected with methylamine in water and (VIIIc) is deprotected with acetic acid.
【1】 Martin, J.C.; Hitchcock, M.J.M.; Starrett, J.E. Jr.; Ghazzouli, I.; Mansuri, M.M.; Ho, H.-T.; Sommadossi, J.-P.; Brankovan, V.; Synthesis, anti-HIV activity, and biological properties of 2',3'-didehydro-2',3'-dideoxythymidine (D4T). Nucleosides Nucleotides 1989, 32, 461-6. |
【2】 Starret, J.E. Jr.; Mansuri, M.M.; Martin, J.C.; Fuller, C.E.; Howell, H.G. (Bristol-Myers Squibb Co.); Production of 2',3'-dideoxy-2',3'-didehydronucleosides. EP 0334368 . |
【3】 Lin, T.-S.; Prusoff, W.H. (Yale University); Pharmaceutical compsn. comprising 3'-deoxythymidin-2'-ene (3'-deoxy-2',3'-didehydrothymidine) in treating patients infected with retrovirus. EP 0273277 . |
【4】 Mansuri, M.M.; Ghazzouli, I.; Starrett, J.E. Jr.; Hitchcock, M.J.; Sterzycki, R.Z.; Brankovan, V.; Lin, T.S.; August, E.M.; Prusoff, W.H.; Sommadossi, J.P.; et al.; 1-(2,3-Dideoxy-beta-D-glycero-pent-2-enofuranosyl)thymine. A highly potent and selective anti-HIV agent. J Med Chem 1989, 32, 2, 461-6. |
【5】 Lin, T.-S.; Gao, Y.-S.; August, E.M.; Prusoff, W.H.; Qian, H.-Y.; Synthesis of [2-14C]3'-deoxythymidin-2'-ene (d4T) and [5-125I]3'-azido-2',3'-dideoxy-5-iodouridine: Potent inhibitors of human immunodeficiency virus (HIV-1). J Label Compd Radiopharm 1989, 27, 6, 669-74. |
【6】 Da Rooge, M.A.; Noel, M.; Horwitz, J.P.; Chua, J.; Klundt, I.L.; Nucleosides. IX. The formation of 2',3'-unsaturated pyrimidine nucleosides via a novel beta-elimination reaction. J Org Chem 1966, 31, 205-11. |
【7】 Chattopadhyaya, J.; Agback, P.; Vial, J.M.; A new synthesis of 1-(2,3-dideoxy-beta-D-glycero-pent-2-enofuranosyl)-thymine. A highly potent and selective anti-HIV agent. Nucleosides Nucleotides 1990, 9, 2, 245-58. |
【8】 Baba, M.; De Clercq, E.; Pauwels, R.; Herdewijn, P.; Balzarini, J.; Vanderhaeghe, H.; Broder, S.; 3'-Substituted 2',3'-dideoxynucleoside analogues as potential anti-HIV (HTLV-III/LAV) agents. J Med Chem 1987, 30, 8, 1270-8. |
【9】 Robinson, C.; Castaner, J.; Stavudine. Drugs Fut 1994, 19, 10, 925. |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(IVa) | 12166 | 1-[(2R,4S,5R)-4-Hydroxy-5-[(trityloxy)methyl]tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione | C29H28N2O5 | 详情 | 详情 | |
(IVb) | 12194 | 1-((2R,4S,5R)-4-Hydroxy-5-[[(4-methoxyphenyl)(diphenyl)methoxy]methyl]tetrahydro-2-furanyl)-5-methyl-2,4(1H,3H)-pyrimidinedione | C30H30N2O6 | 详情 | 详情 | |
(IVc) | 12195 | [(2R,3S,5R)-3-hydroxy-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-2-furanyl]methyl 2-pyridinecarboxylate | C16H17N3O6 | 详情 | 详情 | |
(Va) | 12196 | (2R,3S,5R)-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-2-[(trityloxy)methyl]tetrahydro-3-furanyl methanesulfonate | C30H30N2O7S | 详情 | 详情 | |
(Vb) | 12197 | (2R,3S,5R)-2-[[(4-methoxyphenyl)(diphenyl)methoxy]methyl]-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]tetrahydro-3-furanyl methanesulfonate | C31H32N2O8S | 详情 | 详情 | |
(Vc) | 12198 | [(2R,3S,5R)-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-3-[(methylsulfonyl)oxy]tetrahydro-2-furanyl]methyl 2-pyridinecarboxylate | C17H19N3O8S | 详情 | 详情 | |
(VIa) | 12199 | 5-Methyl-1-[(2R,5S)-5-[(trityloxy)methyl]-2,5-dihydro-2-furanyl]-2,4(1H,3H)-pyrimidinedione | C29H26N2O4 | 详情 | 详情 | |
(VIIb) | 12200 | 1-[(2R,4R,5R)-5-[[(4-Methoxyphenyl)(diphenyl)methoxy]methyl]-4-(phenylselanyl)tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione | C36H34N2O5Se | 详情 | 详情 | |
(VIIc) | 12201 | [(2R,3R,5R)-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-3-(phenylselanyl)tetrahydro-2-furanyl]methyl 2-pyridinecarboxylate | C22H21N3O5Se | 详情 | 详情 | |
(VIIIb) | 12202 | 1-((2R,5S)-5-[[(4-Methoxyphenyl)(diphenyl)methoxy]methyl]-2,5-dihydro-2-furanyl)-5-methyl-2,4(1H,3H)-pyrimidinedione | C30H28N2O5 | 详情 | 详情 | |
(VIIIc) | 12203 | 1-[(2R,5S)-5-(Methoxymethyl)-2,5-dihydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione | C11H14N2O4 | 详情 | 详情 | |
(I) | 12161 | Thymidine; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione | 50-89-5 | C10H14N2O5 | 详情 | 详情 |
(II) | 12191 | ((2R,3S,5R)-5-[5-methyl-2,4-dioxo-3,4-dihydro-1(2H)-pyrimidinyl]-3-[[methyl(methylene)oxo-lambda(6)-sulfanyl]oxy]tetrahydro-2-furanyl)methyl methanesulfonate | C13H20N2O8S2 | 详情 | 详情 | |
(III) | 12192 | 1-[(1R,3R,5R)-2,6-Dioxabicyclo[3.2.0]hept-3-yl]-5-methyl-2,4(1H,3H)-pyrimidinedione | C10H12N2O4 | 详情 | 详情 |