【结 构 式】 |
【药物名称】 【化学名称】N-[4-[2-(2-Amino-4-ethyl-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-L-glutamic acid 【CA登记号】 【 分 子 式 】C22H25N5O5 【 分 子 量 】439.47505 |
【开发单位】Duquesne University (Originator), Tufts University (Originator) 【药理作用】ONCOLYTIC DRUGS, Dihydrofolate Reductase (DHFR) Inhibitors, Thymidylate Synthase Inhibitors |
合成路线1
2-Pivaloylamino-pyrrolo[2,3-d]pyrimidine-4-one (I) is chlorinated to (II) in refluxing POCl3. Subsequent, Stille coupling of (II) with tributyl vinyltin gives the vinyl pyrrolopyrimidine (III), which is further reduced to the corresponding ethyl derivative (IV) by catalytic hydrogenation over Pd/C. Regioselective iodination of (IV) by means of N-iodosuccinimide provides (V). Aryl iodide (V) is then subjected to palladium-catalyzed cross-coupling with trimethylsilylacetylene (VI), leading to the silylethynyl adduct (VII). Desilylation of (VII) employing tetrabutylammonium fluoride furnishes intermediate (VIII).
【1】 Gangjee, A.; Yu, J.; Kisliuk, R.L.; Haile, W.H.; Sobrero, G.; McGuire, J.J.; Design, synthesis, and biological activities of classical N-[4-[2-(2-amino-4-ethylpyrrolo[2,3-d]pyrimidin-5-yl)ethyl] benzoyl]-L-glutamic acid and its 6-methyl derivative as potential dual inhibitors of thymidylate synthase and dihydrofolate reductase and. J Med Chem 2003, 46, 4, 591. |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(I) | 14794 | 2,2-dimethyl-N-(4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-2-yl)propanamide | C11H14N4O2 | 详情 | 详情 | |
(II) | 63357 | N-(4-chloro-7H-pyrrolo[2,3-d]pyrimidin-2-yl)-2,2-dimethylpropanamide | C11H13ClN4O | 详情 | 详情 | |
(III) | 63358 | N-(4-ethenyl-7H-pyrrolo[2,3-d]pyrimidin-2-yl)-2,2-dimethylpropanamide | C13H16N4O | 详情 | 详情 | |
(IV) | 63359 | N-(4-ethyl-7H-pyrrolo[2,3-d]pyrimidin-2-yl)-2,2-dimethylpropanamide | C13H18N4O | 详情 | 详情 | |
(V) | 63360 | N-(4-ethyl-5-iodo-7H-pyrrolo[2,3-d]pyrimidin-2-yl)-2,2-dimethylpropanamide | C13H17IN4O | 详情 | 详情 | |
(VI) | 23897 | ethynyl(trimethyl)silane;trimethylsilyl acetylene | 1066-54-2 | C5H10Si | 详情 | 详情 |
(VII) | 63361 | N-{4-ethyl-5-[2-(trimethylsilyl)ethynyl]-7H-pyrrolo[2,3-d]pyrimidin-2-yl}-2,2-dimethylpropanamide | C18H26N4OSi | 详情 | 详情 | |
(VIII) | 63362 | N-(4-ethyl-5-ethynyl-7H-pyrrolo[2,3-d]pyrimidin-2-yl)-2,2-dimethylpropanamide | C15H18N4O | 详情 | 详情 |
合成路线2
Coupling of acetylene (VIII) with diethyl N-(4-iodobenzoyl)-L-glutamate (IX) gives rise to the disubstituted acetylene (X). This is then hydrogenated to (XI) in the presence of Pd/C. Finally, hydrolysis of (XI) under alkaline conditions leads to the title compound.
【1】 Gangjee, A.; Yu, J.; Kisliuk, R.L.; Haile, W.H.; Sobrero, G.; McGuire, J.J.; Design, synthesis, and biological activities of classical N-[4-[2-(2-amino-4-ethylpyrrolo[2,3-d]pyrimidin-5-yl)ethyl] benzoyl]-L-glutamic acid and its 6-methyl derivative as potential dual inhibitors of thymidylate synthase and dihydrofolate reductase and. J Med Chem 2003, 46, 4, 591. |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(VIII) | 63362 | N-(4-ethyl-5-ethynyl-7H-pyrrolo[2,3-d]pyrimidin-2-yl)-2,2-dimethylpropanamide | C15H18N4O | 详情 | 详情 | |
(IX) | 14283 | diethyl (2S)-2-[(4-iodobenzoyl)amino]pentanedioate | C16H20INO5 | 详情 | 详情 | |
(X) | 63363 | diethyl 2-({[4-(2-{2-[(2,2-dimethylpropanoyl)amino]-4-ethyl-7H-pyrrolo[2,3-d]pyrimidin-5-yl}ethynyl)phenyl]carbonyl}amino)pentanedioate | C31H37N5O6 | 详情 | 详情 | |
(XI) | 63364 | diethyl 2-({[4-(2-{2-[(2,2-dimethylpropanoyl)amino]-4-ethyl-7H-pyrrolo[2,3-d]pyrimidin-5-yl}ethyl)phenyl]carbonyl}amino)pentanedioate | C31H41N5O6 | 详情 | 详情 |