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【结 构 式】 
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【药物名称】JK-1624SO2 【化学名称】23-(tert-Butylsulfonyl)-16,17-dehydro-1alpha-hydroxy-24,25,26,27-tetranorvitamin D3 【CA登记号】 【 分 子 式 】C27H42O4S 【 分 子 量 】462.69739 |
【开发单位】Johns Hopkins University (Originator), M.D. Anderson Cancer Center (Originator) 【药理作用】ONCOLYTIC DRUGS, Vitamin D Analogs |
合成路线1
1-Ethylideneperhydroindan-4-ol (I) reacts with Me2AlCl and paraformaldehyde via an ene process to give the homoallylic diol (II), the primary hydroxyl group of which is tosylated by means of TsCl and DMAP and the secondary hydroxyl group silylated by means of TESOTf and lutidine to yield (III). Conversion of (III) into the corresponding nitrile by means of KCN in DMSO, followed by reduction with DIBALH in toluene, affords the aldehyde (IV) (1), which is further reduced in THF by treatment with DIBALH in hexanes to yield (V). The hydroxy silyl ether (V) is then converted into the iodo derivative (VI) by treatment with PPh3, imidazole and I2 in dichloromethane. The iodo silyl ether (VI) reacts with t-BuSH in the presence of DBU in benzene to afford sulfide (VII), which is then oxidized and deprotected with oxone to provide the hydroxy sulfone (VIII). Oxidation of (VIII) with pyridinium dichromate (PDC) in CH2Cl2 gives the keto sulfone (IX), which reacts with the phosphine oxide derivative (X) in THF in the presence of PhLi. Finally, the protecting silyl ether moiety is removed by means of Bu4NF in THF.
| 【1】 Posner, G.H.; Wang, Q.; Han, G.; et al.; Conceptually new sulfone analogues of the hormone 1alpha,25-dihydroxyvitamin D3: Synthesis and preliminary biological evaluation. J Med Chem 1999, 42, 18, 3425. |
| 【2】 Posner, G.H.; et al.; Noncalcemic, antiproliferative, transcriptionally active, 24-fluorinated hybrid analogues of the hormone 1alpha,25-dihydroxyvitamin D3. Synthesis and preliminary biological evaluation. J Med Chem 1998, 41, 16, 3008. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 42566 | (3aR,4S,7aS)-1-[(Z)ethylidene]-7a-methyloctahydro-1H-inden-4-ol | C12H20O | 详情 | 详情 | |
| (II) | 38682 | (3aS,7S,7aR)-3-[(1S)-2-hydroxy-1-methylethyl]-3a-methyl-3a,4,5,6,7,7a-hexahydro-1H-inden-7-ol | C13H22O2 | 详情 | 详情 | |
| (III) | 42567 | (2S)-2-[(3aS,7S,7aR)-3a-methyl-7-[(triethylsilyl)oxy]-3a,4,5,6,7,7a-hexahydro-1H-inden-3-yl]propyl 4-methylbenzenesulfonate | C26H42O4SSi | 详情 | 详情 | |
| (IV) | 42568 | (3R)-3-[(3aS,7S,7aR)-3a-methyl-7-[(triethylsilyl)oxy]-3a,4,5,6,7,7a-hexahydro-1H-inden-3-yl]butanal | C20H36O2Si | 详情 | 详情 | |
| (V) | 42569 | (3R)-3-[(3aS,7S,7aR)-3a-methyl-7-[(triethylsilyl)oxy]-3a,4,5,6,7,7a-hexahydro-1H-inden-3-yl]-1-butanol | C20H38O2Si | 详情 | 详情 | |
| (VI) | 42570 | ([(3aS,7S,7aR)-3-[(1R)-3-iodo-1-methylpropyl]-3a-methyl-3a,4,5,6,7,7a-hexahydro-1H-inden-7-yl]oxy)(triethyl)silane; (3aS,7S,7aR)-3-[(1R)-3-iodo-1-methylpropyl]-3a-methyl-3a,4,5,6,7,7a-hexahydro-1H-inden-7-yl triethylsilyl ether | C20H37IOSi | 详情 | 详情 | |
| (VII) | 42571 | (3aS,7S,7aR)-3-[(1R)-3-(tert-butylsulfanyl)-1-methylpropyl]-3a-methyl-3a,4,5,6,7,7a-hexahydro-1H-inden-7-yl triethylsilyl ether; ([(3aS,7S,7aR)-3-[(1R)-3-(tert-butylsulfanyl)-1-methylpropyl]-3a-methyl-3a,4,5,6,7,7a-hexahydro-1H-inden-7-yl]oxy)(triethyl)silane | C24H46OSSi | 详情 | 详情 | |
| (VIII) | 42572 | (3aS,7S,7aR)-3-[(1R)-3-(tert-butylsulfonyl)-1-methylpropyl]-3a-methyl-3a,4,5,6,7,7a-hexahydro-1H-inden-7-ol | C18H32O3S | 详情 | 详情 | |
| (IX) | 42573 | (3aR,7aS)-1-[(1R)-3-(tert-butylsulfonyl)-1-methylpropyl]-7a-methyl-3,3a,5,6,7,7a-hexahydro-4H-inden-4-one | C18H30O3S | 详情 | 详情 | |
| (X) | 42574 | [2-((3S,5R)-3,5-bis[[tert-butyl(dimethyl)silyl]oxy]-2-methylenecyclohexylidene)ethyl](oxo)diphenylphosphorane; 2-((3S,5R)-3,5-bis[[tert-butyl(dimethyl)silyl]oxy]-2-methylenecyclohexylidene)ethyl(diphenyl)phosphine oxide | C33H51O3PSi2 | 详情 | 详情 |
合成路线2
The bromination of the Hajos dione (XI) with t-Bu-Cu, DIBAL and Br2 in HMPA/THF gives the bromo derivative (XII), which is reduced with LiAlH(t-BuO)3 in THF to yield the diol (XIII). The dehydrobromination of (XIII) with KH in HMPA affords the epoxide (XIV), which is oxidized with pyridinium dichromate (PDC) in dichloromethane to provide the ketoepoxide (XV). The Wittig condensation of (XV) with phosphonium salt (XVI) by means of potassium tert-butoxide gives the ethylidene epoxide (XVII), which is finally opened with LiAlH4 in THF to yield the target intermediate (I).
| 【1】 Liu, W.; Daniewski, A.R.; A novel silycopper catalyst for the reductivo bromination of hajos dione. Improved preparation of a CD synthon for teh synthesis of vitamin D. J Org Chem 2001, 66, 2, 626. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 42566 | (3aR,4S,7aS)-1-[(Z)ethylidene]-7a-methyloctahydro-1H-inden-4-ol | C12H20O | 详情 | 详情 | |
| (XI) | 45534 | (7aS)-7a-methyl-2,3,7,7a-tetrahydro-1H-indene-1,5(6H)-dione | 17553-86-5 | C10H12O2 | 详情 | 详情 |
| (XII) | 45535 | (3aR,4S,7aS)-4-bromo-7a-methylhexahydro-1H-indene-1,5(4H)-dione | 2706-38-9 | C10H13BrO2 | 详情 | 详情 |
| (XIII) | 45536 | (1S,3aR,4S,5S,7aS)-4-bromo-7a-methyloctahydro-1H-indene-1,5-diol | C10H17BrO2 | 详情 | 详情 | |
| (XIV) | 45537 | (1aS,3aS,4S,6aR,6bR)-3a-methyloctahydro-1aH-indeno[4,5-b]oxiren-4-ol | C10H16O2 | 详情 | 详情 | |
| (XV) | 45538 | (1aS,3aS,6aR,6bR)-3a-methyloctahydro-4H-indeno[4,5-b]oxiren-4-one | C10H14O2 | 详情 | 详情 | |
| (XVI) | 11717 | Ethyl(triphenyl)phosphonium bromide; (Ethyl)triphenylphosphonium bromide | 1530-32-1 | C20H20BrP | 详情 | 详情 |
| (XVII) | 45539 | (1aS,3aS,6aR,6bR)-4-[(Z)ethylidene]-3a-methyloctahydro-4H-indeno[4,5-b]oxirene | C12H18O | 详情 | 详情 |
合成路线3
The silylation of the acetonide (XVIII) with Tms-Cl and LDA in THF gives dienolate (XIX), which is enantioselectively condensed with acrolein (XX) by means of Carreira's Ti catalyst in ethyl ether to yield the chiral allyl alcohol (XXI). The reaction of (XXI), N,O-dimethylhydroxylamine (XXII) and Me2AlCl affords the amide (XXIII), which is selectively reduced with Me4NBH(OAc)3 in HOAc/acetonitrile to provide the dihydroxyamide (XXIV). The silylation of the OH groups of (XXIV) with Tbdms-Cl and imidazole gives the disilylated compound (XXV), which is submitted to a Grignard reaction with vinylmagnesium bromide (XXVI) in THF to yield the vinyl ketone (XXVII). The Michael addition reaction with Ph2P(O)-Li and simultaneous triflation with (XXVIII) in THF affords the enol triflate (XXIX), which is cyclized by means of Pd(OAc)2, PPh3 and TEA in THF to provide the phosphorane (XXXa-b) as an inseparable mixture of the (Z)- and (E)-isomers. Finally, this mixture is submitted to photochemical isomerization with a medium-pressure UV mercury lamp in the presence of 9-fluorenone, furnishing the target intermediate, the (Z)-isomer (X) with a 95 % yield.
| 【1】 Anné, S.; et al.; Enantioselective syntheses of key A-ring precursors of 1alpha,25-dihydroxyvitamin D3 and analogues. Synlett 1999, 9, 1435. |
| 【2】 Hiyamizu, H.; et al.; A concise enantioselective synthesis of a key A-ring synthon for 1alpha-hydroxyvitamin D3 compounds. Org Lett 2001, 3, 3, 473. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (XXXa),(X) | 42574 | [2-((3S,5R)-3,5-bis[[tert-butyl(dimethyl)silyl]oxy]-2-methylenecyclohexylidene)ethyl](oxo)diphenylphosphorane; 2-((3S,5R)-3,5-bis[[tert-butyl(dimethyl)silyl]oxy]-2-methylenecyclohexylidene)ethyl(diphenyl)phosphine oxide | C33H51O3PSi2 | 详情 | 详情 | |
| (XXXb) | 53658 | (E)-2-((3S,5R)-3,5-bis{[tert-butyl(dimethyl)silyl]oxy}-2-methylenecyclohexylidene)ethyl(diphenyl)phosphine oxide; (E)-[2-((3S,5R)-3,5-bis{[tert-butyl(dimethyl)silyl]oxy}-2-methylenecyclohexylidene)ethyl](oxo)diphenylphosphorane | n/a | C33H51O3PSi2 | 详情 | 详情 |
| (XVIII) | 13327 | 2,2,6-Trimethyl-4H-1,3-dioxin-4-one；2,2,6-trimethyl-1,3-dioxin-4-one；2,2,6-trimethyl-m-Dioxin-4-one;3-(1-hydroxy-1-methylethoxy)-d-lactone Crotonicacid | 5394-63-8 | C7H10O3 | 详情 | 详情 |
| (XIX) | 53651 | [(2,2-dimethyl-4-methylene-4H-1,3-dioxin-6-yl)oxy](trimethyl)silane; 2,2-dimethyl-4-methylene-4H-1,3-dioxin-6-yl trimethylsilyl ether | n/a | C10H18O3Si | 详情 | 详情 |
| (XX) | 17668 | acrylaldehyde; Acrolein | 107-02-8 | C3H4O | 详情 | 详情 |
| (XXI) | 53652 | 6-[(2S)-2-hydroxy-3-butenyl]-2,2-dimethyl-4H-1,3-dioxin-4-one | n/a | C10H14O4 | 详情 | 详情 |
| (XXII) | 13361 | (Methoxyamino)methane; N,O-Dimethylhydroxylamine | 1117-97-1 | C2H7NO | 详情 | 详情 |
| (XXIII) | 53653 | (5S)-5-hydroxy-N-methoxy-N-methyl-3-oxo-6-heptenamide | n/a | C9H15NO4 | 详情 | 详情 |
| (XXIV) | 53654 | (3S,5S)-3,5-dihydroxy-N-methoxy-N-methyl-6-heptenamide | n/a | C9H17NO4 | 详情 | 详情 |
| (XXV) | 53655 | (3S,5S)-3,5-bis{[tert-butyl(dimethyl)silyl]oxy}-N-methoxy-N-methyl-6-heptenamide | n/a | C21H45NO4Si2 | 详情 | 详情 |
| (XXVI) | 16524 | bromo(vinyl)magnesium | 1826-67-1 | C2H3BrMg | 详情 | 详情 |
| (XXVII) | 53626 | (4S)-3,3-diethyl-4-{4-[(4-methyl-1-piperazinyl)carbonyl]phenoxy}-2-azetidinone | n/a | C19H27N3O3 | 详情 | 详情 |
| (XXVIII) | 34685 | N-(5-chloro-2-pyridinyl)(trifluoro)-N-[(trifluoromethyl)sulfonyl]methanesulfonamide | 145100-51-2 | C7H3ClF6N2O4S2 | 详情 | 详情 |
| (XXIX) | 53657 | (3S,5S)-3,5-bis{[tert-butyl(dimethyl)silyl]oxy}-1-[(E)-2-(diphenylphosphoryl)ethylidene]-6-heptenyl trifluoromethanesulfonate | n/a | C34H52F3O6PSSi2 | 详情 | 详情 |