【结 构 式】 |
【药物名称】Exatecan mesilate, DX-8951f, DX-8951(free base) 【化学名称】(1S,9S)-1-Amino-9-ethyl-5-fluoro-9-hydroxy-4-methyl-2,3,9,10,13,15-hexahydro-1H,12H-benzo[de]pyrano[3',4':6,7]indolizino[1,2-b]quinoline-10,13-dione mesylate 【CA登记号】169869-90-3, 171335-80-1 (free base), 197720-53-9 (hydrate), 144008-87-7 (monohydrochloride) 【 分 子 式 】C25H26FN3O7S 【 分 子 量 】531.56427 |
【开发单位】Daiichi Pharmaceutical (Originator), Yakult Honsha (Originator), EORTC (Not Determined) 【药理作用】Breast Cancer Therapy, Leukemia Therapy, Myeloid Leukemia Therapy, Oncolytic Drugs, Pancreatic Cancer Therapy, Prostate Cancer Therapy, Camptothecins, DNA Topoisomerase I Inhibitors |
合成路线1
Friedel-Crafts acylation of 2-fluorotoluene (I) with succinic anhydride (II) in the presence of AlCl3 afforded 4-(4-fluoro-3-methylphenyl)-4-oxobutanoic acid (III). Ketone (III) reduction by hydrogenation over Pd/C furnished the arylbutyric acid (IV), which was esterified to (V) by means of SOCl2 in MeOH. Subsequent aromatic ring nitration of (V) yielded (VI). After basic hydrolysis of the methyl ester of (VI), the arylbutyric acid (VII) was subjected to intramolecular cyclization in hot polyphosphoric acid producing tetralone (VIII). Reduction of the keto group of (VIII) with NaBH4, followed by dehydration of the resultant alcohol (IX) under acidic conditions, gave rise to the dihydronaphthalene (X). Hydrogenation of the olefin double bond of (X) and simultaneous nitro group reduction in the presence of PtO2 produced the amino tetralin (XI). The amino group of (XI) was further protected as the acetamide (XII) employing Ac2O and Et3N. Regioselective benzylic oxidation of (XII) with KMnO4 gave tetralone (XIII). Functionalization of the alpha position of (XIII) to ketone was achieved employing n-butyl nitrite and potassium tert-butoxide. The resulting keto oxime (XIV) was then reduced with zinc in the presence of Ac2O, yielding acetamide (XV). Both amide functions of (XV) were hydrolyzed under acidic conditions to give diamine (XVI). The aliphatic amine of (XVI) was then selectively acylated with ethyl trifluoroacetate to produce the trifluoroacetamide (XVII).
【1】 Chilman-Blair, K.; Mealy, N.E.; Castaner, J.; Bayes, M.; Exatecan Mesilate. Drugs Fut 2004, 29, 1, 9. |
【2】 Terasawa, H.; Ejima, A.; Ohsuki, S.; Uoto, K. (Daiichi Pharmaceutical Co., Ltd.; Yakult Honsha Co., Ltd.); Hexacyclic cpd.. EP 0495432; JP 1993059061; US 5834476; US 6407115 . |
【3】 Terasawa, H.; Sato, K.; Mitsui, I. (Daiichi Pharmaceutical Co., Ltd.; Yakult Honsha Co., Ltd.); Antitumor agents. JP 1994087746 . |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(I) | 52759 | 2-Fluorotoluene; o-Fluorotoluene | 95-52-3 | C7H7F | 详情 | 详情 |
(II) | 11291 | Dihydro-2,5-furandione; Succinic anhydride | 108-30-5 | C4H4O3 | 详情 | 详情 |
(III) | 52760 | 4-(4-fluoro-3-methylphenyl)-4-oxobutanoic acid | C11H11FO3 | 详情 | 详情 | |
(IV) | 52761 | 4-(4-fluoro-3-methylphenyl)butanoic acid | C11H13FO2 | 详情 | 详情 | |
(V) | 52762 | methyl 4-(4-fluoro-3-methylphenyl)butanoate | C12H15FO2 | 详情 | 详情 | |
(VI) | 52763 | methyl 4-(4-fluoro-5-methyl-2-nitrophenyl)butanoate | C12H14FNO4 | 详情 | 详情 | |
(VII) | 52764 | 4-(4-fluoro-5-methyl-2-nitrophenyl)butanoic acid | C11H12FNO4 | 详情 | 详情 | |
(VIII) | 52765 | 7-fluoro-8-methyl-5-nitro-3,4-dihydro-1(2H)-naphthalenone | C11H10FNO3 | 详情 | 详情 | |
(IX) | 52766 | 7-fluoro-8-methyl-5-nitro-1,2,3,4-tetrahydro-1-naphthalenol | C11H12FNO3 | 详情 | 详情 | |
(X) | 52767 | 6-fluoro-5-methyl-8-nitro-1,2-dihydronaphthalene | C11H10FNO2 | 详情 | 详情 | |
(XI) | 52768 | 3-fluoro-4-methyl-5,6,7,8-tetrahydro-1-naphthalenylamine; 3-fluoro-4-methyl-5,6,7,8-tetrahydro-1-naphthalenamine | C11H14FN | 详情 | 详情 | |
(XII) | 52769 | N-(3-fluoro-4-methyl-5,6,7,8-tetrahydro-1-naphthalenyl)acetamide | C13H16FNO | 详情 | 详情 | |
(XIII) | 52770 | N-(3-fluoro-4-methyl-8-oxo-5,6,7,8-tetrahydro-1-naphthalenyl)acetamide | C13H14FNO2 | 详情 | 详情 | |
(XIV) | 52771 | N-[3-fluoro-7-(hydroxyimino)-4-methyl-8-oxo-5,8-dihydro-1(6H)-naphthalenyl]acetamide | C13H13FN2O3 | 详情 | 详情 | |
(XV) | 52772 | N-[7-(acetylamino)-3-fluoro-4-methyl-8-oxo-5,6,7,8-tetrahydro-1-naphthalenyl]acetamide | C15H17FN2O3 | 详情 | 详情 | |
(XVI) | 52773 | 2,8-diamino-6-fluoro-5-methyl-3,4-dihydro-1(2H)-naphthalenone | C11H13FN2O | 详情 | 详情 | |
(XVII) | 52774 | N-(8-amino-6-fluoro-5-methyl-1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-2,2,2-trifluoroacetamide | C13H12F4N2O2 | 详情 | 详情 |
合成路线2
The pyranoindolizine acetal (XVIII) was hydrolyzed to the trione (XIX) in 90% trifluoroacetic acid. Condensation of (XIX) with amino ketone (XVII) in refluxing toluene produced the hexacyclic compound (XX) as a diastereomeric mixture. The title compound was then obtained by acidic hydrolysis of the trifluoroacetamido group, followed by isolation of the desired isomer by preparative HPLC. The synthesis of the title compound was also reported by condensation of trione (XIX) with the acetamido tetralone (XXI). The resultant hexacyclic acetamide (XXII) was subsequently hydrolyzed with methanesulfonic acid, and the diastereomeric mixture of mesylate salts was finally separated by fractional crystallization.
【1】 Chilman-Blair, K.; Mealy, N.E.; Castaner, J.; Bayes, M.; Exatecan Mesilate. Drugs Fut 2004, 29, 1, 9. |
【2】 Terasawa, H.; Ejima, A.; Ohsuki, S.; Uoto, K. (Daiichi Pharmaceutical Co., Ltd.; Yakult Honsha Co., Ltd.); Hexacyclic cpd.. EP 0495432; JP 1993059061; US 5834476; US 6407115 . |
【3】 Kamihara, S.; Kanai, K.; Noguchi, S.; Terasawa, H.; Kitaoka, H. (Daiichi Pharmaceutical Co., Ltd.; Yakult Honsha Co., Ltd.); Campthothecin deriv. with antitumour activity. CA 2173671; EP 0737686; JP 1996337584 . |
【4】 Terasawa, H.; Sato, K.; Mitsui, I. (Daiichi Pharmaceutical Co., Ltd.; Yakult Honsha Co., Ltd.); Antitumor agents. JP 1994087746 . |
中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
---|---|---|---|---|---|---|
(XVII) | 52774 | N-(8-amino-6-fluoro-5-methyl-1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-2,2,2-trifluoroacetamide | C13H12F4N2O2 | 详情 | 详情 | |
(XVIII) | 52775 | C15H17NO6 | 详情 | 详情 | ||
(XIX) | 10841 | (4S)-4-Ethyl-4-hydroxy-7,8-dihydro-1H-pyrano[3,4-f]indolizine-3,6,10(4H)-trione | C13H13NO5 | 详情 | 详情 | |
(XX) | 52777 | N-[(9S)-9-ethyl-5-fluoro-9-hydroxy-4-methyl-10,13-dioxo-2,3,9,10,13,15-hexahydro-1H,12H-benzo[de]pyrano[3',4':6,7]indolizino[1,2-b]quinolin-1-yl]-2,2,2-trifluoroacetamide | C26H21F4N3O5 | 详情 | 详情 | |
(XXI) | 52776 | N-(8-amino-6-fluoro-5-methyl-1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)acetamide | C13H15FN2O2 | 详情 | 详情 | |
(XXII) | 52778 | N-[(9S)-9-ethyl-5-fluoro-9-hydroxy-4-methyl-10,13-dioxo-2,3,9,10,13,15-hexahydro-1H,12H-benzo[de]pyrano[3',4':6,7]indolizino[1,2-b]quinolin-1-yl]acetamide | C26H24FN3O5 | 详情 | 详情 |