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【结 构 式】 
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【药物名称】Fadrozole hydrochloride, Fadrozole monohydrochloride, CGS-16949A, Afema, Arensin 【化学名称】(±)-4-(5,6,7,8-Tetrahydroimidazo[1,5-a]pyridin-5-yl)benzonitrile monohydrochloride 【CA登记号】102676-96-0, 102676-47-1 (free base non-specified stereoch.), 102676-31-3 (undefined stereoch.) 【 分 子 式 】C14H14ClN3 【 分 子 量 】259.74078 |
【开发单位】Novartis (Originator) 【药理作用】Breast Cancer Therapy, Oncolytic Drugs, Aromatase Inhibitors |
合成路线1
A new synthesis of fadrozole hydrochloride has been published: The oxidation of 4-(2-pyridyl)benzoic acid ethyl ester (I) with peracetic acid at 90 C gives the corresponding N-oxide (II), which by reaction with dimethyl sulfate and potassium cyanide yields 4-(6-cyano-2-pyridyl)benzoic acid ethyl ester (III). The hydrogenation of (III) with H2 over Pd/C in methanol affords 4-[6-(aminomethyl)-2-pyridyl]benzoic acid ethyl ester (IV), which is formylated with formic acid at 90 C, giving the formamido derivative (V). The cyclization of (V) by means of POCl3 in toluene at 90 C yields 4-(imidazo[1,5-a]pyridin-5-yl)benzoic acid ethyl ester (VI), which is hydrogenated with H2 over Pd/C in anhydrous ethanol containing HCl to afford 4-(5,6,7,8-tetrahydroimidazo[1,5-a]pyridin-5-yl)benzoic acid ethyl ester (VII). The hydrolysis of (VI) with NaOH in ethanol gives the corresponding acid (VIII), which by reaction with refluxing SOCl2 yields the acyl chloride (IX). The reaction of (IX) with dry ammonia in dichloromethane affords the 4-(5,6,7,8-tetrahydroimidazo[1,5-a]pyridin-5-yl)benzamide (X), which is finally treated with POCl3 in refluxing chloroform. The final product is obtained by treatment with dry HCl in ethylether.
| 【1】 Gude, C.; Rodriguez, H.; Steele, R.E.; Browne, L.J.; Fadrozole hydrochloride: A potent, selective, nonsteroidal inhibitor of aromatase for the treatment of estrogen-dependent disease. J Med Chem 1991, 34, 2, 725-36. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 13440 | ethyl 4-(2-pyridinyl)benzoate | C14H13NO2 | 详情 | 详情 | |
| (II) | 13441 | 2-[4-(ethoxycarbonyl)phenyl]-1-pyridiniumolate | C14H13NO3 | 详情 | 详情 | |
| (III) | 13442 | ethyl 4-(6-cyano-2-pyridinyl)benzoate | C15H12N2O2 | 详情 | 详情 | |
| (IV) | 13443 | ethyl 4-[6-(aminomethyl)-2-pyridinyl]benzoate | C15H16N2O2 | 详情 | 详情 | |
| (V) | 13444 | ethyl 4-[6-[(formylamino)methyl]-2-pyridinyl]benzoate | C16H16N2O3 | 详情 | 详情 | |
| (VI) | 13445 | ethyl 4-imidazo[1,5-a]pyridin-5-ylbenzoate | C16H14N2O2 | 详情 | 详情 | |
| (VII) | 13446 | ethyl 4-(5,6,7,8-tetrahydroimidazo[1,5-a]pyridin-5-yl)benzoate | C16H18N2O2 | 详情 | 详情 | |
| (VIII) | 13447 | 4-(5,6,7,8-Tetrahydroimidazo[1,5-a]pyridin-5-yl)benzoic acid | C14H14N2O2 | 详情 | 详情 | |
| (IX) | 13448 | 4-(5,6,7,8-Tetrahydroimidazo[1,5-a]pyridin-5-yl)benzoyl chloride | C14H13ClN2O | 详情 | 详情 | |
| (X) | 13449 | 4-(5,6,7,8-Tetrahydroimidazo[1,5-a]pyridin-5-yl)benzamide | C14H15N3O | 详情 | 详情 |
合成路线2
1) The condensation of 4-(3-hydroxypropyl)-1H-imidazole (I) with dimethylcarbamoyl chloride by means of triethylamine in refluxing acetonitrile followed by reaction with trimethylchlorosilane gives 1-(dimethylcarbamoyl)-4-[3-(trimethylsilyloxy)propyl]imidazole (II), which is condensed with 4-cyanobenzyl bromide (III) in refluxing acetonitrile yielding 1-(4-cyanobenzyl)-5-(3-hydroxypropyl)imidazole (IV). The reaction of (IV) with SOCl2 in refluxing dichloromethane affords the corresponding 3-chloropropyl derivative (V), which is finally cyclized by means of potassium tert-butoxide in THF.
| 【1】 Browne, L.J. (Ciba Geigy Corp.); Substituted imidazo[1,5-A]pyridine derivatives as aromatase inhibitors.. ES 8801262; JP 61012688; US 4617307 . |
| 【2】 Pento, J.T.; Prous, J.; Castaner, J.; CGS-16949A. Drugs Fut 1989, 14, 9, 843. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| 13708 | N,N'-Dimethylcarbamoyl chloride; Dimethylcarbamoyl chloride; [(Chlorocarbonyl)(methyl)amino]methane | 79-44-7 | C3H6ClNO | 详情 | 详情 | |
| (I) | 21245 | 3-(1H-imidazol-4-yl)-1-propanol | C6H10N2O | 详情 | 详情 | |
| (II) | 21246 | N,N-dimethyl-4-[4-(trimethylsilyl)butyl]-1H-imidazole-1-carboxamide | C13H25N3OSi | 详情 | 详情 | |
| (III) | 14200 | 4-(Bromomethyl)benzonitrile; alpha-Bromo-p-tolunitrile | 17201-43-3 | C8H6BrN | 详情 | 详情 |
| (IV) | 21248 | 4-[[5-(3-hydroxypropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H15N3O | 详情 | 详情 | |
| (V) | 21249 | 4-[[5-(3-chloropropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H14ClN3 | 详情 | 详情 |
合成路线3
2) The protection of 4-[3-(ethoxycarbonyl)propyl-1H-imidazole (VI) with trimethylsilyl chloride followed by reduction with diisobutylaluminum hydride (DBAH) in dichloromethane gives 4-(3-formylpropyl)-1-(trimethylsilyl)imidazole (VII), which is condensed with 4-(tert-butylcarbamoyl)bromobenzene (VIII) by means of butyllithium in THF yielding 4-[4-[4-(tert-butylcarbamoyl)phenyl]-4-hydroxybutyl]-1-(trimethylsilyl) imidazole (IX). The reaction of (IX) with refluxing SOCl2 affords 4-[4-chloro-4-(4-cyanophenyl)butyl]-1H-imidazole (X), which is finally cyclized in refluxing CHCl3.
| 【1】 Browne, L.J. (Ciba Geigy Corp.); Substituted imidazo[1,5-A]pyridine derivatives as aromatase inhibitors.. ES 8801262; JP 61012688; US 4617307 . |
| 【2】 Pento, J.T.; Prous, J.; Castaner, J.; CGS-16949A. Drugs Fut 1989, 14, 9, 843. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (VI) | 21250 | ethyl 4-(1H-imidazol-4-yl)butanoate | C9H14N2O2 | 详情 | 详情 | |
| (VII) | 21251 | 4-[1-(trimethylsilyl)-1H-imidazol-4-yl]butanal | C10H18N2OSi | 详情 | 详情 | |
| (VIII) | 21252 | 4-bromo-N-(tert-butyl)benzamide | C11H14BrNO | 详情 | 详情 | |
| (IX) | 21253 | N-(tert-butyl)-4-[1-hydroxy-4-[1-(trimethylsilyl)-1H-imidazol-4-yl]butyl]benzamide | C21H33N3O2Si | 详情 | 详情 | |
| (X) | 21254 | 4-[1-chloro-4-(1H-imidazol-4-yl)butyl]benzonitrile | C14H14ClN3 | 详情 | 详情 |
合成路线4
The synthesis of [14C]-fadrozole hydrochloride has been described: The reaction of 4-bromotoluene (I) with [14C]-copper cyanide in hot DMF gives [14C]-4-methylbenzonitrile (II), which is brominated with N-bromosuccinimide (NBS) and benzoyl peroxide in CCl4 yields the corresponding bromomethyl derivative (III). The condensation of (III) with N,N-dimethyl-4-[3-(trimethylsilyloxy)propyl]imidazole-1-carboxamide (IV) by means of ammonia, followed by desilylation with HCl affords labeled 4-[5-(3-hydroxypropyl)imidazol-1-ylmethyl]benzonitrile (V). The reaction of (V) with SOCl2 in dichloromethane gives the corresponding chloropropyl derivative (VI), which is finally cyclized by means of potassium tert-butoxide in THF and treated with dry HCl in ethanol/ethyl acetate to obtain the corresponding hydrochloride.
| 【1】 Allentoff, A.J.; Chaudhuri, N.K.; Markus, B.; Duelfer, T.; Desai, M.; Synthesis of 14C--labelled CGS 16949A (fadrozole HCl), a potent aromatase inhibitor. J Label Compd Radiopharm 1997, 39, 11, 885. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 14044 | 4-Bromotoluene; 1-Bromo-4-methylbenzene | 106-38-7 | C7H7Br | 详情 | 详情 |
| (II) | 15458 | 4-methylbenzonitrile; p-tolunitrile | 104-85-8 | C8H7N | 详情 | 详情 |
| (II) | 45171 | 4-methylbenzonitrile | C8H7N | 详情 | 详情 | |
| (III) | 14200 | 4-(Bromomethyl)benzonitrile; alpha-Bromo-p-tolunitrile | 17201-43-3 | C8H6BrN | 详情 | 详情 |
| (III) | 45172 | 4-(bromomethyl)benzonitrile | C8H6BrN | 详情 | 详情 | |
| (IV) | 27902 | N,N-dimethyl-4-[3-[(trimethylsilyl)oxy]propyl]-1H-imidazole-1-carboxamide | C12H23N3O2Si | 详情 | 详情 | |
| (V) | 21248 | 4-[[5-(3-hydroxypropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H15N3O | 详情 | 详情 | |
| (V) | 45173 | 4-[[5-(3-hydroxypropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H15N3O | 详情 | 详情 | |
| (VI) | 21249 | 4-[[5-(3-chloropropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H14ClN3 | 详情 | 详情 | |
| (VI) | 45174 | 4-[[5-(3-chloropropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H14ClN3 | 详情 | 详情 |
合成路线5
14C-Radiolabeled fadrozole has been obtained by two similar ways: 1) The condensation of 4-[3-(methanesulfonyloxy)propyl]-N,N-dimethylimidazole-1-carboxamide (VI) with 14C-labeled 4-(bromomethyl)benzonitrile (IX) by means of NH3 followed by hydrolysis with HCl gives radiolabeled 4-[5-(3-hydroxypropyl)imidazol-1-ylmethyl]benzonitrile (X), which is treated with SOCl2 yielding the chloropropyl derivative (XI). Finally, this compound is cyclized to the target compound by means of potassium tert-butoxide. The intermediate compounds imidazole (VI) and radiolabeled benzonitrile (IX) have been obtained as follows: a) Imidazole (VI): The esterification of 3-(4-imidazolyl)-2(E)-propenoic acid (I) with SOCl2 and methanol gives the methyl ester (II), which is reduced with H2 over Pd/C yielding the propionic ester (III). The reduction of (III) with LiAlH4 affords 3-(4-imidazolyl)-1-propanol (IV), which is treated with N,N-dimethyl chloroformamide to afford 4-(3-hydroxypropyl)-N,N-dimethylimidazole-1-carboxamide (V). Finally, this compound is mesylated with methanesulfonyl chloride to give the desired intermediate (VI). b) Radiolabeled benzonitrile (IX): The reaction of 4-bromotoluene (VII) with radiolabeled potassium cyanide gives the radiolabeled 4-methylbenzonitrile (VIII), which is brominated with NBS and benzoylperoxide yielding intermediate (IX).
| 【1】 Allentoff, A.J.; et al.; Palladium-catalyzed aryl cynations in radiosynthesis: Synthesis of 14C-labeled fadrozole, a potent aromatase inhibitor. 218th ACS Natl Meet (Aug 22 1999, New Orleans) 1999, Abst MEDI 49. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 27420 | Urocanic acid; (E)-3-(1H-imidazol-4-yl)-2-propenoic acid | 104-98-3 | C6H6N2O2 | 详情 | 详情 |
| (II) | 39976 | methyl (E)-3-(1H-imidazol-4-yl)-2-propenoate | C7H8N2O2 | 详情 | 详情 | |
| (III) | 39977 | methyl 3-(1H-imidazol-4-yl)propanoate | C7H10N2O2 | 详情 | 详情 | |
| (IV) | 21245 | 3-(1H-imidazol-4-yl)-1-propanol | C6H10N2O | 详情 | 详情 | |
| (V) | 39978 | 4-(3-hydroxypropyl)-N,N-dimethyl-1H-imidazole-1-carboxamide | C9H15N3O2 | 详情 | 详情 | |
| (VI) | 27902 | N,N-dimethyl-4-[3-[(trimethylsilyl)oxy]propyl]-1H-imidazole-1-carboxamide | C12H23N3O2Si | 详情 | 详情 | |
| (VII) | 14044 | 4-Bromotoluene; 1-Bromo-4-methylbenzene | 106-38-7 | C7H7Br | 详情 | 详情 |
| (VIII) | 15458 | 4-methylbenzonitrile; p-tolunitrile | 104-85-8 | C8H7N | 详情 | 详情 |
| (VIII) | 45171 | 4-methylbenzonitrile | C8H7N | 详情 | 详情 | |
| (IX) | 14200 | 4-(Bromomethyl)benzonitrile; alpha-Bromo-p-tolunitrile | 17201-43-3 | C8H6BrN | 详情 | 详情 |
| (IX) | 45172 | 4-(bromomethyl)benzonitrile | C8H6BrN | 详情 | 详情 | |
| (X) | 21248 | 4-[[5-(3-hydroxypropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H15N3O | 详情 | 详情 | |
| (X) | 45173 | 4-[[5-(3-hydroxypropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H15N3O | 详情 | 详情 | |
| (XI) | 21249 | 4-[[5-(3-chloropropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H14ClN3 | 详情 | 详情 | |
| (XI) | 45174 | 4-[[5-(3-chloropropyl)-1H-imidazol-1-yl]methyl]benzonitrile | C14H14ClN3 | 详情 | 详情 |
合成路线6
The esterification of 3-(4-imidazolyl)-2(E)-propenoic acid (I) with SOCl2 and methanol gives the methyl ester (II), which is reduced with H2 over Pd/C yielding the propionic ester (III). The reduction of (III) with LiAlH4 affords 3-(4-imidazolyl)-1-propanol (IV), which is treated with N,N-dimethyl chloroformamide to afford 4-(3-hydroxypropyl)-N,N-dimethylimidazole-1-carboxamide (V). The mesylation of (V) with methanesulfonyl chloride to give the corresponding methanesulfonate (VI), which is condensed with alpha-bromo-4-iodotoluene (XII) by means of NH3 and treated with HCl yielding 3-[1-(4-iodobenzyl)imidazol-5-yl]-1-propanol (XIII). The reaction of (XIII) with SOCl2 affords the 3-chloropropyl derivative (XIV), which is cyclized by means of potassium tert-butoxide to give 5-(4-iodobenzyl)-5,6,7,8-tetrahydroimidazo[1,5-a]pyridine (XV). Finally, this compound is condensed with radiolabeled potassium cyanide by means of palladium tetrakis(triphenylphosphine).
| 【1】 Allentoff, A.J.; et al.; Palladium-catalyzed aryl cynations in radiosynthesis: Synthesis of 14C-labeled fadrozole, a potent aromatase inhibitor. 218th ACS Natl Meet (Aug 22 1999, New Orleans) 1999, Abst MEDI 49. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 27420 | Urocanic acid; (E)-3-(1H-imidazol-4-yl)-2-propenoic acid | 104-98-3 | C6H6N2O2 | 详情 | 详情 |
| (II) | 39976 | methyl (E)-3-(1H-imidazol-4-yl)-2-propenoate | C7H8N2O2 | 详情 | 详情 | |
| (III) | 39977 | methyl 3-(1H-imidazol-4-yl)propanoate | C7H10N2O2 | 详情 | 详情 | |
| (IV) | 21245 | 3-(1H-imidazol-4-yl)-1-propanol | C6H10N2O | 详情 | 详情 | |
| (V) | 39978 | 4-(3-hydroxypropyl)-N,N-dimethyl-1H-imidazole-1-carboxamide | C9H15N3O2 | 详情 | 详情 | |
| (VI) | 27902 | N,N-dimethyl-4-[3-[(trimethylsilyl)oxy]propyl]-1H-imidazole-1-carboxamide | C12H23N3O2Si | 详情 | 详情 | |
| (XII) | 39980 | 1-(bromomethyl)-4-iodobenzene | 16004-15-2 | C7H6BrI | 详情 | 详情 |
| (XIII) | 39979 | 3-[1-(4-iodobenzyl)-1H-imidazol-5-yl]-1-propanol | C13H15IN2O | 详情 | 详情 | |
| (XIV) | 39981 | 5-(3-chloropropyl)-1-(4-iodobenzyl)-1H-imidazole | C13H14ClIN2 | 详情 | 详情 | |
| (XV) | 39982 | 5-(4-iodophenyl)-5,6,7,8-tetrahydroimidazo[1,5-a]pyridine | C13H13IN2 | 详情 | 详情 |
合成路线7
The protection of 3-(1H-imidazol-4-yl)-1-propanol (I) according to known techniques gives (II), which is condensed with 4-iodobenzyl bromide (III) by means of ammonia in acetonitrile, yielding 3-[1-(4-iodobenzyl)-1H-imidazol-5-yl)-1-propanol (IV). The reaction of (IV) with SOCl2 in dichloromethane affords the corresponding chloro derivative (V), which is cyclized by means of LDA and TMEDA in THF, affording 5-(4-iodophenyl)-5,6,7,8-tetrahydroimidazo[1,5-a]pyridine (VI). Finally, this compound is treated with (14C)-KCN and palladium tetrakis(triphenylphosphine) in refluxing THF.
| 【1】 Markus, B.; Jones, L.; Duelfer, T.; Ray, T.; Wu, A.; Allentoff, A.J.; Ciszewska, G.; Palladium-catalyzed aryl cyanations with [C-14]KCN: Synthesis of C-14-labelled fadrozole, a potent aromatase inhibitor. J Label Compd Radiopharm 2000, 43, 11, 1075. |
| 中间体序号 | 中间体编号 | 品名 | CAS号 | 分子式 | 供应商 | 用于合成 |
|---|---|---|---|---|---|---|
| (I) | 21245 | 3-(1H-imidazol-4-yl)-1-propanol | C6H10N2O | 详情 | 详情 | |
| (II) | 47910 | N-(tert-butyl)-4-[3-[(trimethylsilyl)oxy]propyl]-1H-imidazole-1-carboxamide | C14H27N3O2Si | 详情 | 详情 | |
| (III) | 39980 | 1-(bromomethyl)-4-iodobenzene | 16004-15-2 | C7H6BrI | 详情 | 详情 |
| (IV) | 39979 | 3-[1-(4-iodobenzyl)-1H-imidazol-5-yl]-1-propanol | C13H15IN2O | 详情 | 详情 | |
| (V) | 39981 | 5-(3-chloropropyl)-1-(4-iodobenzyl)-1H-imidazole | C13H14ClIN2 | 详情 | 详情 | |
| (VI) | 39982 | 5-(4-iodophenyl)-5,6,7,8-tetrahydroimidazo[1,5-a]pyridine | C13H13IN2 | 详情 | 详情 |